| Description | Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Features :
- White light and fluorescent imaging -
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green). -
Data tools to accelerate research timelines -
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. -
Quickly image a variety of plate formats and cell types -
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types. -
Intelligent analysis with easy-to-use software -
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training. -
High-resolution images -
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days. -
Custom automation options -
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.
... Read More | Filterless readers provide consistent results with steady temperature regulation.
The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Both Filterless readers provide consistent results with steady temperature regulation.
The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. The temperature independent PathCheck sensor automatically normalizes and ensures accurate absorbance values by detecting pipetting errors. Combined with a wide application base, industry-leading SoftMax® Pro Software and validation tools, these readers fit in every lab and every budget.
Pipetting errors eliminated -
This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. It also allows for the elimination of standard curves.
Optimized optical path -
The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. The spectral resolution ensures accuracy of DNA absorbance measurements.
Eight-channel optics -
The optical design of the reader mimics a dual-beam spectrophotometer. Each well has its own sample beam and reference detector. The eight-channel system delivers both precision and speed across the microplate.
Dual wavelength reads -
The reader delivers high performance with round-bottom, flat-bottom or half-area well plates. Dual wavelength readings report the actual absorbance at each wavelength making data analysis convenient.
Flexibility to read a variety of plate formats -
SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate.
Validation made easy -
Verify the accuracy of your absorbance measurements with the SpectraTest® ABS1 Absorbance Validation Plate, using NIST-traceable standards. Testing can be done in your own lab, on your own schedule.
Other features and benefits include: - Eliminate filters
- Get consistent results
- Integrate with ease
... Read More | Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.
With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
User-configurable applications -
User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis -
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
Multi-channel functionality -
Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
Cellular pathway analysis -
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Other features and benefits include:
- Capture flash assays with ease
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Reduce background noise
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Increase fluorescence performance
... Read More | Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.
Configuration changes in minutes -
Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.
Increased dynamic range and sensitivity -
Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.
Other features and benefits include:
- Get automatic Z-height optimization
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Eliminate manual gain adjustment
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Tailor your data analysis
... Read More | Enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility.
Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for Enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility.
Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Never order or replace filters to optimize an assay again!
A patented multichannel optical design mimics a dual-beam spectrophotometer, measures each sample in the plate directly, and eliminates error due to variations in light output between optical fibers on other instruments for more reliable reporting. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time.
The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Additionally, the VersaMax Microplate Reader can be seamlessly integrated with the StakMax® Microlate Handling Stacker through the SoftMax Pro Software.
Features - Enabling Assays without Compromise
- Measuring Accurately and Reproducibly
- Comprehensive Reader Validation
- Automating with Ease
... Read More |