Microplate readers read biological, chemical or physical events that occur on a microtiter plate. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. This makes these readers a staple in research labs specializing in drug discovery, bioassay validation, manufacturing, etc...
Considerations for purchasing a microplate reader
The first thing to consider when purchasing a microplate reader is the detection method. Microplates typically measure absorbance, fluorescence intensity, or luminescence, but models that measure time-resolved fluorescence and fluorescence polarization are also available for purchase. The type of detection to use will depend on the application. ELISAs and protein assays, for example, require absorbance to be measured while luciferase-based gene expression assays will require luminescence to be measured.
Other things to consider include plate format – 96-well and 384-well plates are the norm but higher and lower capacity plate sizes are also available, speed and throughput needed, type of software, and whether or not multiple plates or wells can be read simultaneously.
Find, compare, and request a quote for microplate readers across leading suppliers at Labcompare; including access to product citations, related published figures, and first-hand reviews from our scientific community.
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Company | Agilent Technologies | Agilent Technologies |
Item | Agilent BioTek Synergy H1 Multimode Reader | Agilent BioTek Synergy LX Multimode Reader |
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Catalog Number | Synergy H1 | Synergy LX |
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Description | Agilent BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your Agilent BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection. Fluorescence bandwidth can be set between 9 and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.... Read More | The Agilent BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optic design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein The Agilent BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optic design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford, and cell viability. The data display, output to a USB flash drive, printer, or Gen5 software make Synergy LX a versatile assay workstation. The large touch screen user interface and onboard software simplify programming and save time in operation of microplate and microvolume assays.... Read More |
Optical System | Monochromator or Filter/Dichroics-based | Monochromator or Filter-based |
Measuring Time | 11 to 42 s (varies with wells) | Absorbance - 12 s (96 wells), 23 s (384 wells)
Fluorescence intensity - 24 s (96 wells), 76 s (384 wells) |
Wavelength Range | 230 to 999 nm (depending on model) | 200 nm to 999 nm, in 1 nm increments (Absorbance) 320 to 700 nm (low noise PMT), 320 to 850 nm (red-shifted PMT) |
Plate Formats | 1 to 384 Well Plates | 6 to 384-well plates |
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