When looking for the presence of a specific protein within a sample, an ELISA (enzyme-linked immunosorbent assay) workstation is a good tool to use. ELISA systems are used for medical and botanical diagnostic tests and in industry for quality control. ELISA workstations use two main test types, the indirect ELISA and the sandwich ELISA. In the indirect method, the sample is run over a solid phase to which the antigen of the tested for protein will adhere. An antibody is then washed over the phase, allowing the antibodies to bind to the antigens. A secondary, enzyme-linked antibody is then added, which will interact with the primary antibody to signal where the protein is present. The sandwich method uses the same basic principle, but the sample is added to a plate where antibodies already are, then the secondary antibodies are added, thereby sandwiching the antigen between the two antibodies.
Key considerations for purchasing an ELISA system
There are several variables between ELISA systems, including how small of a sample may be used, how many sample plates it can hold at a time, and the sensitivity of the system.
Click on the links below to discover and compare ELISA systems from various manufacturers:
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| Company | Berthold Technologies GmbH & Co. KG | Berthold Technologies GmbH & Co. KG |
| Item | Crocodile 5-in-one LB 925 ELISA miniWorkstation | Apollo ELISA Reader |
| Catalog Number | 84024 | 57990 |
| Price | | |
| Description | Running your ELISA assays without automation requires an hour or more of your attention, making it difficult to plan other important activities while your assay is being processed. Crocodile 5-in-one provides the same functionality as five individual instruments in a footprint similar to a stand-Running your ELISA assays without automation requires an hour or more of your attention, making it difficult to plan other important activities while your assay is being processed. Crocodile 5-in-one provides the same functionality as five individual instruments in a footprint similar to a stand-alone-reader. Using Crocodile will reduce assay times by eliminating the need to move plates between dispenser, washer, incubator, shaker and reader. This allows you to perform other important activities.... Read More | RELIABLE ELISA AND ABSORBANCE ANALYSIS IN JUST 6 SECONDS
The Apollo ELISA Reader is an intuitive and reliable filter-based microplate reader that can be used for a wide variety of research and routine applications.
The system has been designed to help you accelerate your research combining RELIABLE ELISA AND ABSORBANCE ANALYSIS IN JUST 6 SECONDS
The Apollo ELISA Reader is an intuitive and reliable filter-based microplate reader that can be used for a wide variety of research and routine applications.
The system has been designed to help you accelerate your research combining fast measurement of 96-well plates in just 6 seconds with intuitive 7-inch colour touchscreen stand-alone operation.
Apollo benefits at a glance:
- Wide variety of applications: With its
wavelength range from 340 to 750 nm it
is ideal for ELISA, cytotoxicity assays,
protein colorimetric assays, nucleic acid
quantification (DISCHE assay), endotoxin
assays and more.
- Fast measurement: Read your samples in
just 6 seconds (fast mode).
- Intuitive operation: 7‘’ colour touchscreen
and preprogrammed protocols simplify
operation of the system.
- Trust your data: Accuracy of „± 1 % or
± 0.005 OD (0-3 OD) whichever is greater
(@405 nm)
- Large dynamic range: The system´s
dynamic range of 4.0 OD is large enough
to cover any assay´s requirements.
Applications:
The Apollo is not only a robust and reliable ELISA reader: it's a flexible filter-based absorbance reader, suitable for many enzymatic and colorimetric assays, such as:
- ELISA and other immunoassays
- Reporter gene assays based on ß-galactosidase and alkaline phosphatase
- Protein quantification using Lowry and Bradford
- DNA quantification using the diphenylamine assay
- Custom filters available for additional assays
... Read More |
| Applications | All types of ELISA in a variety of fields, including: Veterinary diagnostics, Food testing, Clinical research, ELISA R&D and QC | Inquire |
| Sample Format | 1 x 96 well microplate (SBS standard) | Inquire |
| Operating Temperature | operation at 15 to 35 °C | Inquire |
| Dimensions | (WxDxH) 26.3 x 62.6 x 25.8 cm | Inquire |
| Wavelength Range | 400 to 690 nm (pre-installed filters: 405, 450, 492, 620 nm) | 340 to 750 nm |
| Detector(s) | 8 independent photometric channels plus reference channel | Inquire |
| Plate Formats | 96 well microplate | 96-well |
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