Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
ItemSCL-40 HPLC/UHPLC System ControllerNexera UCs UHPLC/SFC Switcing SystemNexera UHPLC Method ScoutingNexera XR UHPLC SystemNexera-i MT Method Transfer System
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Catalog NumberSCL-40Nexera UC SwitchingNexera Method ScoutingNexera XRNexera-I MT
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DescriptionThe SCL-40 features a built-in reservoir tray and color LCD touch screen, stacking neatly on top of the main system components. Other LC modules connect via fiber optic cables for easy "plug and play" system configuration. An internal web server offers convenient control and monitoring of the UHPLC ... Read MoreThis system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and efficiency by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read MoreNexera XR is perfect for fast separations using the popular SPP column phases for high efficiency and lower back pressure. This system offers a rugged and forgiving UHPLC workflow, with five to ten-minute separations. With Analytical Intelligence functions, the system makes lab management simple, ... Read MoreEnables Both HPLC and UHPLC Analysis

The Nexera-i MT is a new addition to the i-Series lineup. It enables both conventional analysis via its high compatibility with existing LC systems and rapid analysis via high-speed methods. In pharmaceutical development, a single integrated system allows
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QuantityEAEAEAEAEA
Detector(s)InquireInquireSPD-M20AUV, PDA, ELSD, Fluorescence, RID, ConductivityInquire
Flow RateInquireInquireInquire0.0001 – 3 mL/min (1.0 – 70 MPa); 3 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)Inquire
Max. PressureInquireInquire100 Mpa70 MpaInquire
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