Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
ItemNexera XS UHPLC SystemNexera UCs UHPLC/SFC Switcing SystemNexera UHPLC Method ScoutingNexera X3 UHPLC SystemNexera-i MT Method Transfer System
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Catalog NumberNexera XSNexera UC SwitchingNexera Method ScoutingNexera X3Nexera-I MT
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DescriptionThe Nexera XS UHPLC excels at fast separations, easily handling small ID UHPLC columns with sub-2 micron particles or the popular SPP phases for high efficiency and lower back pressure. A common XS configuration may have a 4-channel solvent selection valve on each pump providing versatility for ... Read MoreThis system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and efficiency by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read MoreNexera X3 excels in high throughput environments requiring fast separations. The system easily handles small ID UHPLC columns with sub-2 micron particles for high efficiency chromatography. Five-minute run times are the norm. With Analytical Intelligence functions, the system makes lab management ... Read MoreEnables Both HPLC and UHPLC Analysis

The Nexera-i MT is a new addition to the i-Series lineup. It enables both conventional analysis via its high compatibility with existing LC systems and rapid analysis via high-speed methods. In pharmaceutical development, a single integrated system allows
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QuantityEAEAEAEAEA
Detector(s)UV, PDA, ELSD, Fluorescence, RID, ConductivityInquireSPD-M20AUV, PDA, ELSD, Fluorescence, RID, ConductivityInquire
Flow Rate0.0001 – 3 mL/min (1.0 – 105 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)InquireInquire0.0001 – 3 mL/min (1.0 – 130 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)Inquire
Max. Pressure105 MpaInquire100 Mpa130 MpaInquire
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