High-performance liquid chromatography (HPLC) systems are used to analyze and/or purify liquid mixtures. Analytical HPLC systems are used in the food safety, pharmaceutical, and manufacturing industries, as well as in medical and research laboratories. Two of the most common types of analytical HPLC systems are normal phase HPLC and reversed phase HPLC. Normal phase uses a polar stationary phase and a non-polar mobile phase to separate components of a mixture, while reversed phase HPLC uses phases with the opposite polarities of normal phase HPLC. The sample liquid is mixed with the mobile phase and run through a column that contains the stationary phase. The way the sample moves through the column is the basis for the analysis.
Considerations for purchasing an Analytical HPLC System
To determine which analytic HPLC system is the best fit for your lab, decide which features will be needed. Multi-solvent or multi-column analysis, stationary phase pore size, possible pump pressures, possible flow rates, and number of wavelength detectors are all factors that can vary.
Discover and compare Analytical HPLC Systems:
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Company | JASCO | JASCO |
Item | LC-4500 Compact HPLC System | LC-4000 Series HPLC System |
Citations | | |
Catalog Number | LC-4500 | LC-4000-35 |
Price | | |
Detector(s) | UV-Vis, Fluorescence, PDA, Multi-Wavelength, Circular Dichroism Detectors | Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer |
Flow Rate | PU-4580: 0.5 uL to 10.0 mL/min | PU-4180: up to 10 mL/min (conventional to semi-prep, up to 10 mm I.D. column) |
Max. Pressure | 50 MPa from 0.5 to 6 mL/min 35MPa up to 10mL/min | 700 Bar (70 MPa) |
Description | The LC-4500 Compact HPLC System is so flexible that it can be used for a wide range of applications in academic research, teaching, or QA/QC.
The extremely compact stackable design requires only 6 inches (15 cm) of bench space, and can be easily fit into most fume cabinets where space is a premiumThe LC-4500 Compact HPLC System is so flexible that it can be used for a wide range of applications in academic research, teaching, or QA/QC.
The extremely compact stackable design requires only 6 inches (15 cm) of bench space, and can be easily fit into most fume cabinets where space is a premium.
The individual pump (PU-4580), detector (UV-4570), and autosampler (AS-4550) modules each have an option for keypad control, external control by RS232, or simple contact closure, so that they can be readily incorporated into other systems for both chromatography and non-chromatography applications.
The system employs an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than the commonly used conventional twin-piston reciprocating design.
In the LC-4500 model, SSQD has been re-developed, with a new solvent delivery mechanism offering the highest stability in solvent delivery across the entire analytical flow rate range.
Features: - Compact - LC-4500 compact module (width: 15 cm) makes effective use of lab space.
- Flexible - PU-4580 enables to configure the various solvent delivery system, and AS-4550 can provide the various sample injection method. Therefore, these modules achieve to build the ideal system. Combination with existing LC-4000 modules can provides wider application.
- User-friendly - For those users who require front-panel control, the LC-4500 model retains the popular keypad and display (with a backlit LED display as standard). Keypad control can still be configured for convenient override when under PC control.
... Read More | The LC-4000 Series HPLC System with maximum operating pressure (350 bar, 35 MPa) has been designed for routine HPLC research and academic settings. The system can be used with conventional 5 µm particle size analytical columns for flow rates of 0.5 to 10 mL/min. |
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