| Description | The ultraDAWN™ instrument provides real-time product attributes for bioprocess monitoring and control. This instrument is a unique multi-angle light scattering (MALS) instrument, designed for monitoring key attributes like aggregation, polymerization, or conjugation of macromolecules and The ultraDAWN™ instrument provides real-time product attributes for bioprocess monitoring and control. This instrument is a unique multi-angle light scattering (MALS) instrument, designed for monitoring key attributes like aggregation, polymerization, or conjugation of macromolecules and nanoparticles such as proteins, biopolymers, gene vectors and nanodrug-delivery vehicles. With its accompanying OBSERVER™ software, ultraDAWN calculates and reports molar mass, size, particle concentration or payload, in real time, up to 300 times per minute. ultraDAWN determines the particle concentration for sample in the range of a molecular weight from 103 to 109 g/mol and radius from 10 to 250 nm. It can also determined the Full-total capsid ratio (Vg/Cp), full and empty titer and size of small viral vectors like AAV. OBSERVER can generate a trigger for controlling the process based on allowed values of a product attribute like particle size or gene vector payload. In addition, the real-time data may be fed back to the process controller for control purposes and saved for post-process analytics. OBSERVER’s trigger functionality can be used to control collection of desirable product, reject out-of-spec material, indicate process endpoints or critical alarms.
Use cases include process development and production. Accelerate PD with immediate feedback on product quality when changing conditions such as flow rates, column loading, resin, buffer conditions or process duration. Or monitor and control the process based on direct measurements of product attributes, without invoking process models; obtain immediate data on product quality for confidence in sending material to the next unit operation.
Key Features:
- In-line operation at 1 - 150 mL/min, with response time delay (RTD) below 1 second.
- On-line configuration for higher flow rates or for drawing continuously from a homogenizer or reactor vessel, with typical RTD of 0.5 – 3.0 minutes
- Optional integration with production control software via OPC-UA
... Read More | The DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar massThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, conjugation (e.g. glycoproteins) or encapsulation (e.g. AAV capsid content). It stands out with exceptional sensitivity and the widest range of molecular weight, size, and concentration of any MALS instrument, paired with a broad selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS), size exclusion chromatography (SEC-MALS) or an EclipseTM, field-flow fractionation instrument (FFF-MALS) and may also be used with ion-exchange chromatography or reverse-phase chromatography. The instrument includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics and is accompanied by ASTRA™, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.... Read More | The microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size andThe microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects, the microDAWN is similar to the well-established miniDAWN, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius. The microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion and produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC. In some cases it may be used with ion-exchange or other types of chromatography.... Read More | Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected sample, 100 kDa polystyrene in THF (varies for other samples and solvents). The miniDAWN is engineered with self-contained modules that are easily replaced making it field serviceable. The system-ready monitors soise levels in real-time and an all-green status indicator lets you know when your system is ready for data collection. The optional DLS module uses the WyattQELS™ module, which fits inside the miniDAWN to providing on-line dynamic light scattering for determining radii down to 0.5 nm. All light scattering cells are subject to contamination by particles and to combat this issue the instrument has a self-cleaning, embedded COMET™ ultrasonic module. For maximum reliability and uptime, program the miniDAWN to automatically clean the cell after every run. The units may also be combined with a Calypso™ composition-gradient system to characterize biomolecular interactions.... Read More |
| Detector(s) | High-gain, high dynamic range photodiodes at 18 detection angles | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS.
Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. | High-gain, high dynamic range, static light scattering detectors at 3 angles | High-gain, high-dynamic range photodiodes at 3 detection angles for MALS. Optional avalanche photodiode for simultaneous dynamic light scattering measurements. |
| Light Source | 658 nm diode laser, progrmmable from 10% to 100% maximum power | 658 nm laser, programmable 10% - 100% of maximum power.
Optional: 785 nm for fluorescence suppression | 660 nm laser, programmable 10% - 100% of maximum power. | 658 nm laser, programmable from 10% to 100% maximum power. |