HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyThermo Fisher ScientificThermo Fisher ScientificThermo Fisher ScientificThermo Fisher ScientificThermo Fisher Scientific
ItemThermo Scientific™ Vanquish™ Fraction CollectorThermo Scientific™ UltiMate™ 3000 Basic Automated SystemThermo Scientific™ UltiMate™ 3000 Binary Standard SD SystemThermo Scientific™ UltiMate™ 3000 Quaternary Rapid Separation RS SystemThermo Scientific™ Vanquish™ Flex Binary UHPLC System
Citations
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Catalog NumberIQLAAAGABHFAPUMZZZIQLAAAGABHFAPBMBFCIQLAAAGABHFAPBMBEXIQLAAAGABHFAPBMBFAIQLAAAGABHFAPUMBJC
Price
TypeIntegrated Fraction CollectorAutomatedBinaryQuaternaryInquire
Flow Rate0.05 mL/min to 10 mL/min10 mL/min10 mL/minInquire8 mL/min
Max. PressureInquire62 Mpa / 620 bar / 9000 psi62 Mpa / 620 bar / 9000 psiup to 1000 bar (15,000psi)up to 1000 bar (15,000psi)
DescriptionExperience compelling fraction purity with the Thermo Scientific™ Vanquish™ Fraction Collector (VFC) using innovative valve technology for enhanced resolution, product recovery, and minimized carry-over. The FC can be used with any analytical flow Thermo Scientific™ Vanquish™... Read MoreThe Thermo Scientific™ UltiMate™ 3000 Automated system offers unique LC and entry level UHPLC capabilities without compromise. The integrated autosampler and thermostatted column compartment deliver retention time precision and the ability to fine tune backpressure, resolution and speed. With ... Read MoreThe Thermo Scientific ™ UltiMate™ 3000 Binary Standard System provides a fast gradient response, excellent flow stability, and a broad choice of high-data-rate detection technologies to ensure reliable results from every sample, making the UltiMate 3000 Binary Standard System an ideal choice for ... Read MoreThe Thermo Scientific™ UltiMate™ 3000 Rapid Separation Quaternary System supports the use of up to four solvents at up to 15,000 psi (100 MPa, 1000 bar) for fast routine applications or method development. With excellent flow stability, a large choice of detection technologies and a recommended flow... Read MoreThe Thermo Scientific™ Vanquish™ Flex Binary UHPLC system is biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending. This ultra high performance liquid chromatography system shares all Vanquish values, such as a design focused on uptime, ... Read More
QuantityEAEAEAEAEA
Capacity4 segments useable for sample racks or well plates with SBS footprint formatInquireInquireInquireInquire
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