HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
Itemi-Series Integrated (u)HPLC SystemsAmino Acid Analysis SystemCannabis Analyzer for PotencyLC-40 HPLC SystemNexera UHPLC Method Scouting
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Catalog Numberi-Series LC-50/60Amino Acid HPLCCannabis AnalyzerLC-40Nexera Method Scouting
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TypeAnalyticalInquireInquireHPLCUHPLC
Detector(s)UV/VIS, PDA, RI, Fluorescence, ELSDInquireInquireUV, PDA, ELSD, Fluorescence, RID, ConductivitySPD-M20A
Flow Rate0.0001 to 10 mL/minInquireInquire0.0001 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)Inquire
Max. Pressure70 MPaInquireInquire44 Mpa100 Mpa
DescriptionBuilding on the exceptional performance of previous versions, the integrated i-Series LC-2050/LC-2060 models incorporate Analytical Intelligence functions, enable complete automation from start-up to analysis preparations, and allow remote monitoring and data processing. In addition, the easy-to-use... Read MoreThe analysis of amino acids plays an important role not only in the food industry, but also in natural product and pharmaceutical fields. This system uses detection by post-column fluorescence derivatization, with o-phthalaldehyde (OPA) / N-acetylcysteine as the reaction reagent, to selectively and ... Read MoreThe Shimadzu Cannabis Analyzer for Potency captures the spirit of an Analyzer – a comprehensive package integrating instrument hardware, software, consumables, and analytical workflow. Includes a certified reference material (CRM) mixture of target compounds. Also includes a service package to cover... Read MoreThe modular LC-40 system is the workhorse for everyday HPLC, whether using traditional 5 micron fully porous LC columns or the popular SPP column phases for high efficiency separations with lower back pressures. With Analytical Intelligence functions, the system makes lab management simple, enabling... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read More
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