HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
Itemi-Series Integrated (u)HPLC Systems2D HPLC for Bioanalysis (Co-Sense for BA)CBM-40 HPLC/UHPLC System ControllerNexera UHPLC Method ScoutingNexera-i MT Method Transfer System
Citations
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Catalog Numberi-Series LC-50/60Co-Sense for BACBM-40Nexera Method ScoutingNexera-I MT
Price
TypeAnalyticalAnalyticalInquireUHPLCInquire
Detector(s)UV/VIS, PDA, RI, Fluorescence, ELSDUV, LCMSInquireSPD-M20AInquire
Flow Rate0.0001 to 10 mL/minInquireInquireInquireInquire
Max. Pressure70 MPaInquireInquire100 MpaInquire
DescriptionBuilding on the exceptional performance of previous versions, the integrated i-Series LC-2050/LC-2060 models incorporate Analytical Intelligence functions, enable complete automation from start-up to analysis preparations, and allow remote monitoring and data processing. In addition, the easy-to-use... Read MoreAutomatically and seamlessly conducting all processes from sample pre-treatment to analysis, the Prominence 2D HPLC for BA is a column-switching HPLC system equipped with the newly developed Shim-pack MAYI-ODS pretreatment column and a unique on-line dilution bypass channel. The system’s on-line pre... Read MoreThe CBM-40 is a small-profile unit that stacks neatly with main system components. Other LC modules connect via fiber optic cables for easy "plug and play" system configuration. An internal web server offers convenient control and monitoring of the UHPLC system through a web browser, LabSolutions ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read MoreEnables Both HPLC and UHPLC Analysis

The Nexera-i MT is a new addition to the i-Series lineup. It enables both conventional analysis via its high compatibility with existing LC systems and rapid analysis via high-speed methods. In pharmaceutical development, a single integrated system allows
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