GPC Refractive Index Detectors are one of the most commonly used detectors in gel permeation chromatography/size-exclusion chromatography. GPC RI detectors measure the refractive index of eluted compounds as they pass through the chromatographic column by comparing the sample to the pure solvent. The measured refractive index is directly proportional to its concentration, allowing the detector to provide quantitative data about the sample. When comparing GPC refractive index detectors ensure that the measurement range and sensitivity are sufficient for your assay as these can directly affect analysis and vary between models. For laboratories performing protein or polymer analysis, ensure that the temperature range is appropriate for your samples. When using a GPC refractive index detector always be sure to use high-quality solvents to achieve the best sensitivity. Thanks to their wide availability and good balance between sensitivity and ease of use, GPC RI detectors are an excellent option for most size-exclusion chromatography applications.
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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | Optilab™ Refractive Index Detector for HPLC/GPC | microOptilab™ Refractive Index Detector for UHPLC |
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| Catalog Number | Optilab | microOptilab |
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| Description | The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same wavelength as the MALS detector, for maximum accuracy. With an operating window from 4°C - 65°C it is appropriate for both protein and polymer samples. Key benefits include its extraordinary range: -0.0047 to +0.0047 RIU (comparable to 25 mg/mL protein in PBS); the high-concentration version extends the range by 7x. Its sensitivity is ± 7.5 x 10-10 RIU across the entire measurement range. It also includes a high-resolution, touch-screen front panel that displays data graphs and diagnostics.... Read More | The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4.7 x 10-3 to + 4.7 x 10-3 RIU (comparable to 25 mg/mL protein in PBS). The detector is highly sensitive, ± 1.5 x 10-9 RIU, across the entire measurement range. This detector measures solvent refractive index, required for MALS calculations at the same wavelength as the MALS detector, for maximum accuracy. The temperature range of 4°C - 65°C is appropriate for both proteins and polymers that require a range of temperatures. It also features a high-resolution, touch-screen front panel displays data graphs, system health indicators and instrument diagnostics.... Read More |
| Principle | Inquire | UHPLC Compatible |
| Light Source | 658 nm ± 20 nm LED (488 nm, 785 nm and 940 nm wavelengths are available in the high-concentration version) | 660 nm standard (other wavelengths available) |
| Cell Volume | 7.4 µL | 7.4 µL |
| Dimensions | (WxDxH) 36 x 58 x 18 cm | (WxDxH) 36 x 58 x 18 cm |
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