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| Company | FUJIFILM Wako Chemicals U.S.A. Corporation | FUJIFILM Wako Chemicals U.S.A. Corporation | FUJIFILM Wako Chemicals U.S.A. Corporation | FUJIFILM Wako Chemicals U.S.A. Corporation | FUJIFILM Wako Chemicals U.S.A. Corporation |
| Item | EpiSight Bisulfite Conversion Kit | 2,2'-Azobis(2-amidinopropane)Dihydrochloride | Honokiol Standard | NIP Standard (Nitrophen) | S. cerevisiae Direct Transformation Kit |
| Citations | | | | | |
| Catalog Number | 297-71901 | 992-11062 | 083-08511 | 144-03931 | 292-62703 |
| Price | Supplier Page | Supplier Page | Supplier Page | Supplier Page | Supplier Page |
| Description | EpiSight Bisulfite Conversion Kit (Wako Cat. No. 297-71901) offers a fast and efficient alternative to traditional bisulfite conversion. The protocol is optimized to minimize reagent preparation and bisulfite conversion time. This kit contains Enhancer for bisulfite conversion to allow the EpiSight Bisulfite Conversion Kit (Wako Cat. No. 297-71901) offers a fast and efficient alternative to traditional bisulfite conversion. The protocol is optimized to minimize reagent preparation and bisulfite conversion time. This kit contains Enhancer for bisulfite conversion to allow the enhancement of the bisulfite conversion efficiency and inhibit of DNA degradation.
Subsequent PCR-amplification using EpiSight BisulTaq DNA Polymerase*, recombinant, Solution (Wako Cat. No. 297-72001) is more efficient compared to using conventional Taq DNA polymerase.
Features: - Minimizing DNA damage
- High conversion rate of Cytosine to Uracil
- Adoption of Wako's unique Enhancer
- Efficient PCR amplification of bisulfite treated-DNA
*Hot-Start Gene Taq NT (Nippon Gene)(Wako Cat. #311-07523 (250 units) is also recommended, depending on DNA samples.... Read More | 2,2’-Azobis(2-amidinopropane) Dihydrochloride is a water soluble highly efficient polymerization initiator. It decomposes to give cationic free radicals in solution at relatively low temperatures. Melting Point: 160 – 169°C Pkg: 25g | Natural Ingredient Standard Source: Mangolia sp. F.W.: 266.34 Pkg: 20 mg | Application: Agriculture Standard Formula: C12H7Cl2NO3 F.W.: 284.09 CAS No. [1836-75-5] Melting Point: 71~73 °C Pkg: 200 mg | This kit is designed for easy transformation of the budding yeast, Saccharoyces sp.. The protocol allows for successful transformation simply by mixing a plasmid DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The kit isThis kit is designed for easy transformation of the budding yeast, Saccharoyces sp.. The protocol allows for successful transformation simply by mixing a plasmid DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The kit is particularly well suited for high-throughput transformation of a large number of yeast strains grown in 96-well plates. Even genome-wide screening, which has been probematic, becomes possible, since the kit easily transforms about 4,850 strains with gene disruption.... Read More |
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