| Description | Many imaging experiments require the ability to move to multiple locations in a chamber or in a tissue culture dish. Large motorized stages accomplish this, but generally make the microscope into a single-use, motorized imaging station. The form factor of motorized stages usually precludes Many imaging experiments require the ability to move to multiple locations in a chamber or in a tissue culture dish. Large motorized stages accomplish this, but generally make the microscope into a single-use, motorized imaging station. The form factor of motorized stages usually precludes positioning other equipment such as manipulators at the same microscope.
Now, a simple attachment converts a standard Sutter MP-285 manipulator into the 3-Dimensional Motorized Stage. The 3DMS manipulator and stage attachment can be either mounted on an MT-75 gantry stand next to an upright microscope, or on an MD series stand attached to the body of an inverted microcope. The compact size allows manipulators and other equipment to be used simultaneously.
Refocusing is often required at the different X-Y locations when images are automatically acquired. Normally this requires a motorized focus drive to be added to the microscope. Because the 3DMS is a three-axis manipulator, focusing (vertical movement of the stage) is already built in. Surprisingly, this relatively small package still allows for 25mm of movement in X, Y, and Z axes with micron precision. Features: 3DMS - Highly stable for experiments intolerant of pipette drift.
- Submicron resolution and integrated coarse positioning.
- 1 inch of motorized travel on all three axes.
- Adjustable speed and resolution allows optimization for your experimental setup.
- When used with MP-285 controller, has programmable robotics for complex motion sequences.
- Continuous display (in microns) of axes positions.
- Switch between continuous or single step movement.
- Absolute and relative origins.
- Remote computer control
- Compact design easily adaptable to your setup.
... Read More | The MP-78 Large Moving Stage is available for patch slice or in vivo experiments where the microscope body cannot be moved to view different fields of view. This is most common in two-photon experiments where the excitation beam path must enter a fixed microscope. In order to visit wide-spaced The MP-78 Large Moving Stage is available for patch slice or in vivo experiments where the microscope body cannot be moved to view different fields of view. This is most common in two-photon experiments where the excitation beam path must enter a fixed microscope. In order to visit wide-spaced fields of view, and potentially position pipettes at wide-spaced locations, the preparation and any associated manipulators must move together on a large stage.
The MP-78 was introduced at the 2007 Imaging Structure and Function in the Nervous System at Cold Spring Harbor Labs. The stage was very well received. The MP-78 is also appropriate for use with inverted microscopes or other scopes that are larger and not easily translated.
The cast aluminum top plate used in the MP-78 has an attractive shape with an extensive 1/4-20 threaded hole pattern on 1-inch centers. This hole pattern is appropriate for mounting Sutter MP-285 or MP-225 manipulator mechanicals using the mounting adapter plates that come with these devices. Optional rotating bases (285RBI) are also compatible and add flexibility. Finally, the new, MP-265/M mechanical can be mounted on its narrow linear slide.
The large-stage platforms provide solid, stable support and ample space to attach manipulators and other instruments as needed. The rigid aluminum top plate sits firmly on two of our well-known MT-75 gantry-style stands. These stands provide height adjustment and sturdy attachment to the user’s vibration table. Multiple degrees of freedom accommodate a variety of microscope and instrument arrangements.
In the center of the MP-78 top plate is a 4X6 inch milled pocket that will accommodate a variety of aluminum stage inserts, designed to secure various commercial tissue chambers or slide holders. Custom aluminum inserts can also be provided. An available magnetic stainless steel insert carries commercial chambers or can be provided with a simple large, through hole for mounting a custom chamber. The magnetic insert allows small magnetic devices (perfusion lines, ground electrodes, small manipulators) to be mounted easily near the dish. Features - Stable support and solid design
- Accomodates a variety of stage inserts
- Compatible with Nikon, Olympus, Zeiss and Leica upright and inverted microscopes
- MP-78 is suitable for:
Multi-site, two-photon, electrophysiology
Multi-site, inverted (or other large microscope) electrophysiology ... Read More | The MT-2000/MT-2078 is a motorized microscope translator system including multiple platforms for manipulators and specimen. Two general styles are available. In the classic design (MT-2000), an independent stand, the MT-75 is used for each manipulator and a similar stand, the MT-150 holds the tissueThe MT-2000/MT-2078 is a motorized microscope translator system including multiple platforms for manipulators and specimen. Two general styles are available. In the classic design (MT-2000), an independent stand, the MT-75 is used for each manipulator and a similar stand, the MT-150 holds the tissue chamber. A new style (MT-2078) uses a single large, fixed stage, the MT-78-FS, to support multiple manipulators and the sample. The classic MT-2000 features free-standing columns that provide rock solid mounting for the tissue recording chamber and multiple micromanipulators. Many prefer this methodology as it maximizes working room for adjusting the microscope and changing pipettes. Furthermore, the independent stand design minimizes movement-related cross talk between manipulators. The MT-2078 single stage has the advantage that it is easy to add additional manipulators and other devices into the design as the manipulator/chamber support is one large platform.
The core of the MT-2000/MT-2078 system is the MT-800 stepper-motor driven X-Y translation table. The motors can be driven by two-axis versions of either the popular MP-285 manipulator controller or the dual manipulator controller MPC-200-ROE. A two-axis rotary optical encoder (ROE) accepts user input to the motorized translator and can be placed in any convenient location in your setup. Either controller gives the user smooth, high-resolution manual control of motion. The MP-285 controller offers extensive on-board robotic functionality and a serial computer interface for PC control. The MPC-200-ROE controller offers a USB computer interface for PC control. Systems using the MP-285 controller are called MT-2000 or MT-2078 while systems using the MPC-200-ROE controller are designated MT-2200 or MT-2278.
Our controller design has allowed Sutter to easily add a focus drive to the MT-2000/MT-2200/MT-2078/MT-2278 systems. The Sutter focus drive employs a direct-coupled stepper motor for lock-step focus movement and adjustable end of travel sensors to limit the travel of the microscope objective to a safe range for the setup. In the focus drive version, MT2000/FD, the ROE input device has inputs for X, Y and Z focus.
MT-800 motorized translators are currently available for the Olympus BX-51WI, Nikon FN-1, Zeiss Axioskop 2 FS and the Leica DMLFS. Focus drives are available for the Olympus BX51WI. Other microscopes can be supported. MT-75, MT-150 and MT-78 stands and stages are compatible with all scopes.... Read More | The MT-1000/MT-1078 is a microscope translator, fixed-stage and manipulator platform for moving optical pathway electrophysiology. Two general styles are available. In the classic design (MT-1000), an independent stand is used for each manipulator and a similar stand holds the tissue chamber. A new The MT-1000/MT-1078 is a microscope translator, fixed-stage and manipulator platform for moving optical pathway electrophysiology. Two general styles are available. In the classic design (MT-1000), an independent stand is used for each manipulator and a similar stand holds the tissue chamber. A new style (MT-1078) uses a single large, fixed stage that is used for multiple manipulators and for the sample as well.
MT-1000: the classic modular design features free-standing columns that provide rock solid mounting for the tissue recording chamber (MT-150) and multiple micromanipulators (MT-75). The columns are positioned around a movable table that provides X-Y translation of the optical pathway in order to view multiple locations within the chamber at high resolution. Many prefer this methodology as it maximizes working room for adjusting the microscope and changing pipettes. Furthermore, the independent stand design minimizes movement-related cross talk between manipulators.
In the new MT-1078 translator system, the large fixed stage MT-78-FS is employed. In this design, it is easy to add additional manipulators and other devices to expand a setup as the manipulator/chamber support is a single large platform. Furthermore, an available magnetic stainless steel insert holds the chamber and allows the easy positioning of magnetically attached small devices nearby.
At the core of both the MT-1000 and MT-1078 translator systems is the MT-500 X-Y translation table. It is designed to allow movement of a microscope’s optics with respect to the fixed stage(s). This methodology was developed by electrophysiologists recording from tissue slice preparations. The translator allows visualization and/or imaging of multiple tissue locations while maintaining multiple stable recordings from the preparation. Such a system is also useful for experiments on cells in culture where one wishes to monitor several cells not in the same field of view, e.g. recording from pre and post synaptic neurons in culture and/or imaging one cell while recording from or stimulating another.
Translators are available for the Olympus BX-51WI, Nikon FN-1, Zeiss Axioskop 2 FS and the Leica DMLFS as well as older fixed stage models. Inverted microscope translators are available for the Olympus IX71.
Various different recording chambers can be accommodated in either the MT-150 chamber column or the insert in the MT-78-FS large stage. Sutter currently supports the Warner Instruments/Harvard Apparatus Series 20 chambers and the ALA 35mm chambers. Others can be accommodated. Dovetail slides for the MT-150 or inserts for the MT-78-FS are available with generic openings for users who wish to develop custom chambers. ... Read More | Designed for experiments where a large single stage is dictated, the MT-78-FS large-stage platform provides solid, stable support and ample space to attach manipulators and other instruments as needed. The rigid aluminum top plate sits firmly on two of our well-known MT-75 gantry-style stands. These Designed for experiments where a large single stage is dictated, the MT-78-FS large-stage platform provides solid, stable support and ample space to attach manipulators and other instruments as needed. The rigid aluminum top plate sits firmly on two of our well-known MT-75 gantry-style stands. These stands provide height adjustment and sturdy attachment to the user’s vibration table. Multiple degrees of freedom accommodate a variety of microscope and instrument arrangements.
The MT-78-FS Fixed Stage platform, paired with a Sutter Instrument XY translator (either the motorized MT-800 or manual MT-500), offers a large, single-platform solution for researchers performing experiments requiring X-Y translation of the optical pathway in order to view multiple locations within the specimen at high resolution. Many prefer a large stage design as it lends itself to the easy addition of manipulators, perfusion systems and other equipment to the setup.
The cast aluminum top plate used in the MT-78-FS has an attractive shape with an extensive 1/4-20 threaded hole pattern on 1-inch centers. This hole pattern is appropriate for mounting Sutter MP285 or MP225 manipulator mechanicals using the mounting adapter plates that come with these devices. Optional rotating bases (285RBI) are also compatible and add flexibility. Finally, the new, MP265/M mechanical can be mounted on its narrow linear slide.
In the center of the MT-78-FS top plate is a 4X6 inch milled pocket that will accommodate a variety of aluminum stage inserts, designed to secure various commercial tissue chambers or slide holders. Custom aluminum inserts can also be provided. An available magnetic stainless steel insert carries commercial chambers or can be provided with a simple large, through hole for mounting a custom chamber. The magnetic insert allows small magnetic devices (perfusion lines, ground electrodes, small manipulators) to be mounted easily near the dish. Features - Stable support and solid design
- Accomodates a variety of stage inserts
- Compatible with Nikon, Olympus, Zeiss and Leica upright and inverted microscopes
- Optional XY translation available (motorized or manual)
- MT-78-FS version compatible with Sutter MT-500 or MT-800 X-Y translators
- MT-78-FS is suitable for:
Multi-site electrophysiology where there is a need for a single large stage ... Read More |