Spectrophotometer

Spectrophotometers are used to measure the concentration of a known substance in a solution. They do this by passing a light through the substance and measuring light intensity as a function of wavelength. Either single beam or double beam spectrophotometers can be purchased. In a single beam spectrophotometer, a reference standard is used to measure light intensity before and after the sample is loaded. For double beam spectrophotometers the beam is split with one beam passing through the standard and the other through the sample to compare intensities. While single beam spectrophotometers are typically less expensive and have higher sensitivity, double beam spectrophotometers tend to be more stable and easier to use. Spectrophotometers are important pieces of equipment in chemistry, physics, biochemistry, clinical, and materials testing labs.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
ItemCary 4000 UV-Vis SpectrophotometerCary 3500 Compact UV-Vis SpectrophotometerCary 3500 Flexible UV-Vis SpectrophotometerCary 7000 Universal Measurement Spectrophotometer (UMS)Cary Eclipse Fluorescence Spectrophotometer
Citations
Reviews
Catalog Number10079200Cary 3500 CompactCary 3500 FlexibleCary 700010075200
Price
TypeUV-VisUV-Vis double beam spectrophotometerUV-Vis double beam spectrophotometerUniversal measurement spectrophotometerFluorescence spectrophotometer
Wavelength Range175 to 900 nm190 to 1100 nm190 to 1100 nm175 to 3300 nm200–900 nm
Wavelength AccuracyInquire± 0.2 nm± 0.2 nmUV-vis ± 0.08 nm,NIR ± 0.4 nm± 1.5 nm
Scanning SpeedUV-Vis 2,000 nm/minUV-Vis 150,000 nm/minUV-Vis 150,000 nm/minUV-Vis 2000 nm/min, NIR 8000 nm/min24,000 nm/min
Spectral BandwidthUV-Vis 0.01 - 5.00 nm0.1 to 5 nm0.1 to 5 nmUV-Vis 0.01 to 5.00 nm; NIR 0.04 to 20 nmExcitation: 1.5, 2.5, 5, 10, 20 nm and 10 nm round. Emission: 1.5, 2.5, 5, 10, 20 nm and 10 nm round
Optical SystemDouble BeamDouble out-of-plane Littrow monochromatorDouble out-of-plane Littrow monochromatorDouble out-of-plane Littrow monochromatorCzerny-Turner monochromator
Light SourceTungsten halogen visible and deuterium arc UVXenon flash lampXenon flash lampTungsten halogen visible and deuterium arc UVXenon Flash Lamp
ResolutionInquire0.1 nm limiting resolution0.1 nm limiting resolutionInquireInquire
DescriptionThe Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.

Controlled by the Cary WinUV software,
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The Cary 3500 Compact UV-Vis is a double-beam spectrophotometer featuring new and unique measurement capabilities that can be deployed in regulated environments. The system performs ambient and temperature-controlled experiments with air-cooled Peltier temperature control, and it accurately analyzes... Read MoreThe Cary 3500 Flexible UV-Vis is a double-beam spectrophotometer with superior photometric performance—for challenging measurements with minimal sample preparation—that can be deployed in regulated environments. The system offers a uniquely large sample compartment with a small footprint for ... Read MoreThe Agilent Cary 7000 universal measurement spectrophotometer (UMS) will satisfy all your solid sampling needs. Collect hundreds of UV-Vis-NIR spectra overnight or characterize optical components and thin films in minutes to hours rather than hours to days. The Cary 7000 UMS is a turn-key solution ... Read MoreThe Agilent Cary Eclipse fluorescence spectrophotometer is sensitive, accurate, and flexible. Ideal for use as a routine fluorometer, the Cary Eclipse can easily be switched between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Additional flexibility can be ... Read More
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