Spectrophotometer

Spectrophotometers are used to measure the concentration of a known substance in a solution. They do this by passing a light through the substance and measuring light intensity as a function of wavelength. Either single beam or double beam spectrophotometers can be purchased. In a single beam spectrophotometer, a reference standard is used to measure light intensity before and after the sample is loaded. For double beam spectrophotometers the beam is split with one beam passing through the standard and the other through the sample to compare intensities. While single beam spectrophotometers are typically less expensive and have higher sensitivity, double beam spectrophotometers tend to be more stable and easier to use. Spectrophotometers are important pieces of equipment in chemistry, physics, biochemistry, clinical, and materials testing labs.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
ItemCary 4000 UV-Vis SpectrophotometerCary 3500 Flexible UV-Vis SpectrophotometerCary 3500 Multicell UV-Vis SpectrophotometerCary 60 UV-Vis SpectrophotometerCary Eclipse Fluorescence Spectrophotometer
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Catalog Number10079200Cary 3500 FlexibleCary 3500 MulticellCary 6010075200
Price
TypeUV-VisUV-Vis double beam spectrophotometerUV-Vis double beam spectrophotometerUV-VisibleFluorescence spectrophotometer
Wavelength Range175 to 900 nm190 to 1100 nm190 to 1100 nm190 to 1100 nm200–900 nm
Wavelength AccuracyInquire± 0.2 nm± 0.2 nm± 0.5 at 541.94 nm± 1.5 nm
Scanning SpeedUV-Vis 2,000 nm/minUV-Vis 150,000 nm/minUV-Vis 150,000 nm/min24,000 nm/min, 80 data points/sec24,000 nm/min
Spectral BandwidthUV-Vis 0.01 - 5.00 nm0.1 to 5 nm0.1 to 5 nm1.5 nm fixedExcitation: 1.5, 2.5, 5, 10, 20 nm and 10 nm round. Emission: 1.5, 2.5, 5, 10, 20 nm and 10 nm round
Optical SystemDouble BeamDouble out-of-plane Littrow monochromatorDouble out-of-plane Littrow monochromatorDouble beamCzerny-Turner monochromator
Light SourceTungsten halogen visible and deuterium arc UVXenon flash lampXenon flash lampXenon flash lampXenon Flash Lamp
ResolutionInquire0.1 nm limiting resolution0.1 nm limiting resolutionInquireInquire
DescriptionThe Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.

Controlled by the Cary WinUV software,
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The Cary 3500 Flexible UV-Vis is a double-beam spectrophotometer with superior photometric performance—for challenging measurements with minimal sample preparation—that can be deployed in regulated environments. The system offers a uniquely large sample compartment with a small footprint for ... Read MoreThe Cary 3500 Multicell UV-Vis is a double-beam spectrophotometer offering new, unique measurement capabilities that can be deployed in regulated environments. The system simultaneously measures up to four independent temperature experiments across eight cuvette positions, with air-cooled Peltier ... Read MoreThe Cary 60 UV-Vis spectrophotometer has a wavelength range of 190–1100 nm that can be scanned in under three seconds. Our flexible UV-Vis system can be fitted with long pathlength cuvettes and solid sample transmission or reflectance accessories, and is ideal for remote UV-Vis absorbance analysis, ... Read MoreThe Agilent Cary Eclipse fluorescence spectrophotometer is sensitive, accurate, and flexible. Ideal for use as a routine fluorometer, the Cary Eclipse can easily be switched between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Additional flexibility can be ... Read More
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