Spectrophotometer

Spectrophotometers are used to measure the concentration of a known substance in a solution. They do this by passing a light through the substance and measuring light intensity as a function of wavelength. Either single beam or double beam spectrophotometers can be purchased. In a single beam spectrophotometer, a reference standard is used to measure light intensity before and after the sample is loaded. For double beam spectrophotometers the beam is split with one beam passing through the standard and the other through the sample to compare intensities. While single beam spectrophotometers are typically less expensive and have higher sensitivity, double beam spectrophotometers tend to be more stable and easier to use. Spectrophotometers are important pieces of equipment in chemistry, physics, biochemistry, clinical, and materials testing labs.

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CompanyShimadzuShimadzuShimadzuShimadzu
ItemUV-2700i Research-Grade UV-Vis SpectrophotometerBioSpec-nano Small Volume SpectrophotometerUV-1900i UV-VIS SpectrophotometerUV-3600i Plus UV-VIS-NIR Spectrophotometer
Citations
Catalog NumberUV-2700iBioSpec-nanoUV-1900iUV-3600i Plus
Price
TypeUV/VisUV VISUV/VisInquire
Wavelength Range185 to 900 nm220 to 800 nm190 to 1100 nm185 to 3,300 nm
Wavelength Accuracy+/-0.1 nm (656.1 nm D2), +/-0.3 nm (all range)Inquire± 0.1 nm at D2 peak 656.1 nm, ± 0.3 nm for entire rangeInquire
Scanning Speed4000 to 0.5 nm/minInquire3000 to 2 nm/min, 29000 nm/min when survey scanningInquire
Spectral Bandwidth0.1, 0.2, 0.5, 1, 2 or 5 nm3 nm1 nmInquire
Optical SystemDouble Beam, Double MonochromatorSingle BeamDouble beamHigh-performance double monochromator
Light Source50 W Halogen Lamp, Deuterium LampXenon flash lampInquireInquire
Resolution0.1 nm+/- 1 nm1 nmMaximum resolution: 0.1 nm
DescriptionThe compact UV-2700i is a universal, research-grade spectrophotometer that can be used in a wide range of fields, and easily expanded to suit the measurement objective. The UV-2700i is capable of 8-Abs measurements, and optimal for measuring low transmittance samples.

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The Shimadzu BioSpec-nano is a low-maintenance micro-volume spectrophotometer designed for the modern life science laboratory. It offers superior detection limits, up to 10 times better compared to the competition, making it the perfect instrument for quantitation of DNA, RNA, protein analysis, and

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Shimadzu’s UV-1900i double-beam spectrophotometer offers an exceptional balance of performance and value. The combination of high resolution, low stray light, and an ultrafast scan function with an easy-to-use interface makes this system ideal for R&D and QC laboratories in a variety of fields, such... Read MoreIncorporating the latest technology to achieve high sensitivity, high resolution and an ultra-low stray light level, Shimadzu’s UV-3600i Plus UV-VIS-NIR spectrophotometer offers scientists an opportunity to provide high-performance analyses in a wealth of application areas, including: materials ... Read More
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