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Spectrofluorometers are designed to detect and quantify fluorophores. These are fluorescent molecules that, when exposed to light, absorb photons at a characteristic wavelength. Subsequently, they then emit photons at a different and slightly longer characteristic wavelength. Spectrofluorometers contain excitation and emission filters for using different fluorophores. Monitoring the activities of molecules labeled with fluorophores is crucial to many kinds of experiments in cell biology, molecular biology and genetics, pharmaceuticals, and forensics, among many others. Emitted light may be detected by photomultiplier tubes or photodiodes within the spectrofluorometer, depending on the model. In addition, users of spectrofluorometers now have a wide range of high-tech fluorophores from which to choose to complement the features of a Spectrofluorometer, also referred to as fluorimeter. Modern fluorometers and fluorimeters are capable of detection as low as 1 part per trillion.
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| Company | Edinburgh Instruments Ltd | Edinburgh Instruments Ltd |
| Item | LifeSpec-II Dedicated Lifetime Spectrometer | FS5 Spectrofluorometer |
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| Catalog Number | LifeSpec-II | FS5 |
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| Light Source | EPLs (Picosecond Pulsed Diode Lasers), EPLEDs (Picosecond Pulsed LEDs) | 150 W Xenon lamp, laser diodes and LEDs |
| Wavelength Range | 200 to 1700 nm | Excitation Wavelength 185 to 1000 nm; Emission Wavelength 200 to 1650 nm |
| Wavelength Accuracy | Inquire | +/- 0.5 nm |
| Scanning Speed | Inquire | 80 nm/s |
| Description | The LifeSpec-II is a compact, high performance computer controlled instrument for the acquisition and analysis of time resolved fluorescence, based on the technique of Time Correlated Single Photon Counting (TCSPC).
The instrument has been designed for operation with modern picosecond and The LifeSpec-II is a compact, high performance computer controlled instrument for the acquisition and analysis of time resolved fluorescence, based on the technique of Time Correlated Single Photon Counting (TCSPC).
The instrument has been designed for operation with modern picosecond and femtosecond lasers, such our EPLs (Picosecond Pulsed Diode Lasers), EPLEDs (Picosecond Pulsed LEDs), Titanium Sapphire lasers and supercontinuum sources. These sources enable measurements of fluorescence lifetimes down to tens of picoseconds across the UV-Visibile-NIR spectral regions up to 1700nm.
The LifeSpec-II has the unique feature of having an integrated emission monochromator with zero temporal dispersion. As such, very short fluorescence lifetimes can be recovered without an error introduced by the propagation of light on wavelength dispersive elements, such as the monochromator gratings.
The integrated system features a large sample compartment, with room for many sample holder options, including multi-position sample holders, cryostats, X-Y position sample holders, etc.
The instrument further comprises of computer controlled attenuation of incident light from the laser, optional polarisers, two computer selectable laser input ports and two computer controlled detector exit ports.
A detector shutter is both interlocked to the sample chamber for protection against excessive ambient light, and is also linked to the detector current to protect against overexposure by the laser.
The LifeSpec-II comes in two versions. The first version contains one set of gratings for the operation in the spectral range 200nm to 1000nm. The second version contains two sets of gratings for the operation in the spectral range 200nm to 1700nm.... Read More | The FS5 is a fully integrated steady state fluorescence spectrometer designed to meet the highest specifications in the research and analytical markets. Ultimate sensitivity, coupled with high speed data acquisition and ease of use, makes the FS5 the ideal plug and play analytical tool. The optical The FS5 is a fully integrated steady state fluorescence spectrometer designed to meet the highest specifications in the research and analytical markets. Ultimate sensitivity, coupled with high speed data acquisition and ease of use, makes the FS5 the ideal plug and play analytical tool. The optical design is the best in class, utilizing specially selected optics to achieve the maximum in signal throughput.
Features
- Using single photon counting techniques for the highest detection sensitivity, the FS5 delivers data you can trust with no need for training.
- Single Photon Counting for the highest sensitivity
- Plug & Play Sample Modules for easy setup and flexibility
- All modes of operation, analysis and presentation controlled by ONE software package for ease of use
- Power saving features as standard - lamp powers down when not in use
- Ultrafast data acquisition for steady state & lifetime
- Two emission ports and NIR upgradeability makes the FS5 unique in its class
- 225mm focal length monochromators for high resolution and maximum throughput
- Water Raman SNR >4000:1
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