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Spectrofluorometers are designed to detect and quantify fluorophores. These are fluorescent molecules that, when exposed to light, absorb photons at a characteristic wavelength. Subsequently, they then emit photons at a different and slightly longer characteristic wavelength. Spectrofluorometers contain excitation and emission filters for using different fluorophores. Monitoring the activities of molecules labeled with fluorophores is crucial to many kinds of experiments in cell biology, molecular biology and genetics, pharmaceuticals, and forensics, among many others. Emitted light may be detected by photomultiplier tubes or photodiodes within the spectrofluorometer, depending on the model. In addition, users of spectrofluorometers now have a wide range of high-tech fluorophores from which to choose to complement the features of a Spectrofluorometer, also referred to as fluorimeter. Modern fluorometers and fluorimeters are capable of detection as low as 1 part per trillion.
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Company | Edinburgh Instruments Ltd | Edinburgh Instruments Ltd |
Item | LifeSpec-II Dedicated Lifetime Spectrometer | FLS1000 Photoluminescence Spectrometer |
Citations | | |
Catalog Number | LifeSpec-II | FLS1000 |
Price | | |
Light Source | EPLs (Picosecond Pulsed Diode Lasers), EPLEDs (Picosecond Pulsed LEDs) | 450 W Ozone-free Xenon Arc Lamp, Microsecond Flashlamp, Picosecond pulsed Diode Lasers (EPLs) and pulsed LEDs (EPLEDs) |
Wavelength Range | 200 to 1700 nm | Emission Wavelength Range 160 nm to 5500 nm |
Wavelength Accuracy | Inquire | 0.2 nm |
Scanning Speed | Inquire | Inquire |
Description | The LifeSpec-II is a compact, high performance computer controlled instrument for the acquisition and analysis of time resolved fluorescence, based on the technique of Time Correlated Single Photon Counting (TCSPC).
The instrument has been designed for operation with modern picosecond and The LifeSpec-II is a compact, high performance computer controlled instrument for the acquisition and analysis of time resolved fluorescence, based on the technique of Time Correlated Single Photon Counting (TCSPC).
The instrument has been designed for operation with modern picosecond and femtosecond lasers, such our EPLs (Picosecond Pulsed Diode Lasers), EPLEDs (Picosecond Pulsed LEDs), Titanium Sapphire lasers and supercontinuum sources. These sources enable measurements of fluorescence lifetimes down to tens of picoseconds across the UV-Visibile-NIR spectral regions up to 1700nm.
The LifeSpec-II has the unique feature of having an integrated emission monochromator with zero temporal dispersion. As such, very short fluorescence lifetimes can be recovered without an error introduced by the propagation of light on wavelength dispersive elements, such as the monochromator gratings.
The integrated system features a large sample compartment, with room for many sample holder options, including multi-position sample holders, cryostats, X-Y position sample holders, etc.
The instrument further comprises of computer controlled attenuation of incident light from the laser, optional polarisers, two computer selectable laser input ports and two computer controlled detector exit ports.
A detector shutter is both interlocked to the sample chamber for protection against excessive ambient light, and is also linked to the detector current to protect against overexposure by the laser.
The LifeSpec-II comes in two versions. The first version contains one set of gratings for the operation in the spectral range 200nm to 1000nm. The second version contains two sets of gratings for the operation in the spectral range 200nm to 1700nm.... Read More | The FLS1000 Spectrometer sets the standard in both steady state and time-resolved photoluminescence spectroscopy for both fundamental research and routine laboratory applications.
The system is a modular fluorescence and phosphorescence spectrometer for measuring spectra from the ultraviolet to The FLS1000 Spectrometer sets the standard in both steady state and time-resolved photoluminescence spectroscopy for both fundamental research and routine laboratory applications.
The system is a modular fluorescence and phosphorescence spectrometer for measuring spectra from the ultraviolet to the mid-infrared spectral range (up to 5,500 nm), and lifetimes spanning from picoseconds to seconds. All of this can be achieved through various upgrade routes, either at the time of order or in the future.
Whether you are studying photophysics, photochemistry, biophysics, biochemistry, material or life sciences, the FLS1000 will enable you to reliably and accurately measure luminescence spectra and kinetics using state-of-the-art sources, detectors, acquisition techniques, quality optics and precision mechanics. The large sample chamber will house practically any type of sample accessory.
High sensitivity is a prerequisite for measurements of low sample concentrations, small sample volumes or low sample quantum yields. The instrument’s guaranteed sensitivity of >35,000:1 for the standard water Raman measurement using the SQRT method is unmatched in the industry.
The FL1000 has a USB interface and all modes of operation are controlled by ONE data acquisition module and ONE all-inclusive Fluoracle software package for data acquisition and analysis. Light source, detector, grating, slits, polarisers are all computer-controlled for accurate and precise measurements.
Features: - New Fluoracle® software package – easily accessible user interface and updated features
- Higher Resolution – new 325 mm focal length monochromators provide superb accuracy and excellent stray light rejection with minimum step size
- Faster measurements – increased scan speeds and leading performance
- Increased automation – ease of use for even the most complex configurations
... Read More |
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