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Most basic recommended instrument:
Cecil Adept System 4S.
It normally takes about 40 minutes to
analyze a 16-component mixture of
polyaromatic hydrocarbons (PAHs)
or polynuclear aromatic hydrocarbons
(PNAs). With the aid of the Cecil
Instruments WaveQuest UV-VIS
HPLC detector, this analysis is greatly
accelerated up to 4 min, and can now
be performed on a routine basis. The
WaveQuest detector is very useful in
checking for the spectral purity of the
fast eluting peaks.
Minimum analytical instrument
requirement- Binary HPLC system featuring an
ultrafast scanning UV-VIS HPLC
detector
- Chromatography system manager with
the ability to visualize spectral data.
Typical method
Temperature: Ambient
Mobile phase: Binary gradient of
water (HPLC grade) and acetonitrile
(HPLC grade) Typical chromatogram:
Gradient: time 0.00 min—50% water,
50% acetonitrile, flow rate 3 mL/min;
time 1.00 min—50% water, 50% acetonitrile,
flow rate 3 mL/min; time
2.50 min—2% water, 98% acetonitrile,
flow rate 3 mL/min; time 4.00
min—2% water, 98% acetonitrile, flow
rate 3 mL/min
Wavelength: 254 nm.
Sample volume: 2 μL.
Run time: 4 min.
Column: 5 cm Supelcosil® LC-PAH
column (Sigma-Aldrich, St. Louis,
MO), 3 μm.
Composition of sample: made up to parts-per-billion levels, of each of the following analyticalgrade components, in acetonitrile (HPLC grade):
Naphthalene: Benzo(a)anthracene
Acenaphthylene: Chrysene
Acenaphthene: Benzo(b)fluoranthene
Fluorene: Benzo(k)fluoranthene
Phenanthrene: Benzo(a)pyrene
Anthracene: Dibenzo(a,h)anthracene
Fluoranthene: Benzo(ghi)perylene
Pyrene: Indo(1,2,3-cd)pyrene.
Cecil Instruments Ltd., Milton Technical Centre,
Cambridge CB4 6AZ, U.K.; tel.: +44 01223
420821; fax: +44 01223 420475; e-mail: info@
cecilinstruments.com; www.cecilinstruments.com.
This note acts purely as a guide to the method.
Cecil Instruments Ltd. takes no responsibility for
the outcome of any analysis.