Millicell® Hanging Cell Culture Insert, PET 0.4 µm, 12-well, 48/pk MCHT12H48 from MilliporeSigma

Product Details
ItemMillicell® Hanging Cell Culture Insert, PET 0.4 µm, 12-well, 48/pk
CompanyMilliporeSigma
PriceSupplier Page
Catalog NumberMCHT12H48
Quantity48
ApplicationsCell Culture

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MilliporeSigma

6211 El Camino Real

Carlsbad, CA 92009

United States

Phone: +17607106100

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Website: sigmaaldrich.com/

(6) Fabrication of Human Skin Equivalents Using Decellularized Extracellular MatrixCurrent ProtocolsMarch 9, 2022Atiya M. Sarmin, John T. Connelly2020) 12‐well plates (ThermoFisher, Nunc 150628)12‐well transwell inserts, 0.4‐mm PTFE (Merck Millipore, MCHT12H48)Cloning rings (Bel‐Art, 378470300)Cell culture incubator, 37°C, 5% CO2 (ThermoFisher, Heracell, 11666310) Preparing

(7) Osthole Regulates Secretion of Pro-Inflammatory Cytokines and Expression of TLR2 and NF-κB in Normal Human Keratinocytes and FibroblastsJournal of Inflammation ResearchMarch 1, 2022Natalia Kordulewska, Justyna Topa, Anna Cieślińska, Beata Jarmołowskainserts with a pore size of 0.4 µm and surface area of 1.13 cm2 (Merck St. Louis, MO, USA, cat. no. MCHT12H48). When the cells reached confluence, the medium for keratinocytes (Lifeline Cell Tech LLC) with low

(8) Osthole Inhibits Expression of Genes Associated with Toll-like Receptor 2 Signaling Pathway in an Organotypic 3D Skin Model of Human Epidermis with Atopic DermatitisCellsDecember 28, 2021Natalia Karolina Kordulewska, Justyna Topa, Robert Stryiński, Beata Jarmołowskadirectly to the center of each MilliCell 0.4 μm pore insert (Millipore, Burlington, MA, USA, cat. no. MCHT12H48) in a 12-well plate (Greiner Bio-One, Kremsmünster, Austria, cat. no. 665110) and incubated at 37 °C

(9) NAMPT-derived NAD+ fuels PARP1 to promote skin inflammation through parthanatos cell deathPLoS biologyNovember 1, 2021Martínez-Morcillo FJ, Cantón-Sandoval J, Martínez-Navarro FJ, Cabas I, Martínez-Vicente I, Armistead J, Hatzold J, López-Muñoz A, Martínez-Menchón T, Corbalán-Vélez R, et al.and further processed with Leica software. Human organotypic 3D models Insert transwells (Merck, MCHT12H48) were seeded with 105 human foreskin keratinocytes (Ker-CT, ATCC CRL-4048) on the transwells in 300

(10) Modulatory Effects of Osthole on Lipopolysaccharides-Induced Inflammation in Caco-2 Cell Monolayer and Co-Cultures with THP-1 and THP-1-Derived MacrophagesNutrientsDecember 31, 2020Natalia K. Kordulewska, Justyna Topa, Małgorzata Tańska, Anna Cieślińska, Ewa Fiedorowicz, Huub F. J. Savelkoul, Beata Jarmołowskaonto 0.4 µm pore inserts with an effective growth area of 1.1 cm2 (Merck St. Louis, MO, USA, cat. no. MCHT12H48) in concentrations of 1.5 × 105 cells/cm2 . Cells were cultured for 14 days in complete DMEM, and the

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