Millicell® Cell Culture Insert, 12 mm, polycarbonate, 0.4 µm PIHP01250 from MilliporeSigma

Product Details
ItemMillicell® Cell Culture Insert, 12 mm, polycarbonate, 0.4 µm
CompanyMilliporeSigma
PriceSupplier Page
Catalog NumberPIHP01250
Quantity50
ApplicationsCell Culture

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MilliporeSigma

6211 El Camino Real

Carlsbad, CA 92009

United States

Phone: +17607106100

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Website: sigmaaldrich.com/

(1) Epithelial-Immune Cell Crosstalk Determines the Activation of Immune Cells In Vitro by the Human Cathelicidin LL-37 at Low Physiological ConcentrationsBiomoleculesAugust 28, 2023Ivan V. Bogdanov, Maria A. Streltsova, Elena I. Kovalenko, Alexander M. Sapozhnikov, Pavel V. Panteleev, Tatiana V. Ovchinnikovacells/cm2 onto 24-well Millicell cell culture inserts (PC, 0.4-μm pore size, 0.6 cm2 surface area, cat. #PIHP01250) (Millipore, Burlington, MA, USA), precoated with 0.2% bovine gelatin (Sigma-Aldrich, Saint Louis, MO,

(2) Commensal Cutibacterium acnes induce epidermal lipid synthesis important for skin barrier functionSci AdvAugust 18, 2023Samia Almoughrabie, Laura Cau, Kellen Cavagnero, Alan M. O'Neill, Fengwu Li, Andrea Roso-Mares, Carine Mainzer, Brigitte Closs, Matthew J. Kolar, Kevin J. Williams, et al.epidermis was obtained by seeding 3 × 105 NHEKs into culture inserts (Sigma-Aldrich, St. Louis, MO; PIHP01250). Cells were cultured submerged in complete EpiLife medium with 2 mM Ca2+ (Thermo Fisher Scientific,

(3) Development of a novel air–liquid interface airway tissue equivalent model for in vitro respiratory modeling studiesScientific ReportsJune 22, 2023Timothy Leach, Uma Gandhi, Kimberly D. Reeves, Kristina Stumpf, Kenichi Okuda, Frank C. Marini, Stephen J. Walker, Richard Boucher, Jeannie Chan, Laura A. Cox, et al.OTE design. 2D ALI culture HBEs were seeded on 0.4 μm pore size Millicell inserts (Millipore Sigma, PIHP01250, Billerica, MA) coated with collagen-I at a density of 4.15 × 105 cells/cm2. The cells were cultured

(4) A versatile, bioengineered skin reconstruction device designed for use in austere environmentsFrontiers in Bioengineering and BiotechnologyJune 8, 2023Joachim G. S. Veit, Morgan Weidow, Monica A. Serbanscaffolds were pre-soaked in fibroblast growth media for 1 h and placed in cell culture inserts (Millipore PIHP01250) to keep the scaffold surface above the surface of the media. A 5 µL drop containing 4.0 × 105 fibroblasts

(5) Proteolytic Activity of the Paracaspase MALT1 Is Involved in Epithelial Restitution and Mucosal HealingInternational journal of molecular sciencesApril 17, 2023Wittner L, Wagener L, Wiese JJ, Stolzer I, Krug SM, Naschberger E, Jackstadt R, Beyaert R, Atreya R, Kühl AA, et al.(Millicell®, Cell culture inserts, 0.4 µm PCF, Merck Millipore Ltd., Carrigtwohill, Ireland; Cat. No. PIHP01250) were fixed with 1% PFA (Roth, Karlsruhe, Germany, Cat. No. 0964.2) in PBS+Mg/Ca, permeabilized using

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