Not Very Effective Concentration Filters

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Company:

Millipore

Product Name:

Amicon Ultra-15, PLHK, membrane Ultracel-PL, 100 kD

Catalog Number:

UFC910024

We opted for these 100KDa membranes to concentrate soluble protein of about 180KDa. We were quite confident following the 90% IgG (150KDa) recovery rate assured by the manufacturer, but the results were very deceiving and we lost a lot of time before noticing that most of the protein flowed through the membrane instead of being retained.

Application :

Filtration/electroporation

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