Buffer for Immunofluorescent Staining

I use this cell staining buffer to resuspend cells for flow cytometry. I have found that cells are stable in this buffer for 2-3 hours on ice. The buffer is ready to use and could be used in immunofluorescent staining of viable or fixed single cell suspensions. The buffer has added serum which helps cells to maintain viability which is essential for my cell sorting experiments. I would highly recommend this buffer for flow cytometry.