Product Name:
Seahorse XF Cell Mito Stress Test Kit
Catalog Number:
103015-100
The energy metabolism of microglia activated with lipid biomolecule was measured using Agilent Seahorse XF analyzer. Serial addition of inhibitors of mitochondrial electron transport chain (Oligomycin, FCCP, Antimycin/Rotenone were added) to study the oxygen consumption rate (OCR). The seahorse assay is a convenient, easy, and quick-to-use method to study energy metabolism. The assay however needs a completely confluent cell layer which can be a little tricky and needs optimization.
Application :
Energy metabolism studies