Lisha Joshi

Good Cell Freezing Container
Tuesday, March 05, 2024
Mr Frosty is a convenient and reusable object to cryopreserve cells. This container gives a freezing rate of -1 °C/min. I use it to freeze secondary cell lines. It has the capacity to store 12 cryovials at a time, which makes it useful. Also, the lid has a very good grip which ensures easy closing ... read more
Excellent Protein Detection System for Western Blot
Friday, February 16, 2024
Odyssey CLx is based on infrared-based method to detect proteins for western blot. It detects 2 wavelengths simultaneously (700 and 800 nm excitation wavelength), which gives an added advantage of detecting two targets on the same membrane at the same time. The instrument is easy to use, very ... read more
Good Method to Study Mitochondrial Respiration
Monday, February 12, 2024
The energy metabolism of microglia activated with lipid biomolecule was measured using Agilent Seahorse XF analyzer. Serial addition of inhibitors of mitochondrial electron transport chain (Oligomycin, FCCP, Antimycin/Rotenone were added) to study the oxygen consumption rate (OCR). The seahorse ... read more
Easy Glycolysis Assay Kit
Monday, February 12, 2024
Energy metabolism of microglia were studied with glycolysis assay kit. This kit measures the lactate secreted in the media by the cells treated with various substances. The enzymatic reaction of lactate results in pyruvate and NADH. This is measured in terms of the intensity of dye color. This in ... read more

Articles by Lisha Joshi