Phosphopeptide enrichment is one of the most challenging sample preparation steps for LC/MS analysis due to the variation in reproducibility and selectivity caused by manual sample preparation. The LC/MS analysis of the phosphopeptide samples can be challenging due to a variety of factors discussed in this application note.

In the Phosphopeptide Challenge of the Human Proteome Project (HPP), participating laboratory received two sample vials of phosphorylated peptides mixed with their non-phosphorylated counterparts and are asked to perform peptide sequence analysis using different methods. Learn how the Agilent AssayMAP Bravo platform provided a fully automated and highly selective phosphopeptide enrichment workflow using high-capacity Fe(III)-NTA cartridges to overcome these challenges.

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