The product line Animaltype consists of multiplex PCR test kits specially developed for the genotyping of breeding livestock from faeces, of foodstuff, tissue or other complex samples for proof-of-origin in meat products and generally for quality management in food industry.
Animaltype Pig is the first commercially available test kit for fast and reliable genotyping of pigs. It includes eleven tetranucleotide-markers. For the most important breeding stocks German Large White (LW), German Landrace (GL), and “Piétrain” (Pi) the combined Power of Exclusion (cPE) of these STR-Markers is equivalent or even better then the 15 dinucleotide-STRs recommended for Germany or Austria.
In one PCR reaction, the eleven polymorphic tetranucleotide Short Tandem Repeats loci 387A12F, S0655, SBH1, SBH2, SBH4, SBH10, SBH13, SBH18, SBH19, SBH20, and SBH22 as well as the gender-specific marker SBH23 are amplified simultaneously. Additionally, the test kit is provided with an allelic ladder and Control DNA that allow standardisation of allele designation between different laboratories.
Preferentially, Animaltype Pig is employed for fast and reliable DNA genotyping of blood or tissue samples (especially ear cartilage). One primer for each locus is fluorescence-labelled with 6-FAM (SBH2, SBH18, SBH4, S0655), HEX (SBH23, SBH20, SBH1, SBH10) or NED (SBH13, 387A12F, SBH22, SBH19) whereas a well-balanced intensity of all signals was elaborated for the primer mix.
The detection limit of Animaltype Pig test kit is less than 10 ng genomic DNA. We recommend to apply 1-10 ng DNA template.
The test kit is recommended for the following applications:
- Proof of origin according the EU-Directive
- Kinship testing in context with control of breeding
- Status of inbreeding for herd book populations