- Pre-packaged lentiviral particles containing GFP- & RFP-tagged proteins of cell structure, autophagy, apoptosis and neuroscience
- Detects the presence or absence of proteins and their subcellular location in live cells
- Enables real-time visualization and tracking of protein movement
Billerica, MA—October 18, 2011—EMD Millipore, the Life Science division of Merck KGaA of Darmstadt, Germany, today launched a suite of LentiBrite™ lentiviral biosensors, pre-packaged lentiviral particles encoding proteins fluorescently tagged with green or red fluorescent protein (GFP or RFP).
The initial group of biosensors will encode proteins responsible for cell structure such as actin and tubulin, a central marker of autophagy (degradation of a cell’s own components) – LC3, and proteins that mark the onset of apoptosis (programmed cell death). Future offerings will focus on neuroscience and nuclear proteins. The biosensors enable detection of the presence or absence of a particular protein, as well as the subcellular location of the protein in live cells under various states either by fluorescent microscopy or time-lapse video capture.
Use of lentivirus to deliver fluorescently-tagged proteins into cells offers higher efficiencies of transfection as compared with traditional chemical-based and other non-viral-based methods. In addition, the virus particles can be used to transfect dividing, non-dividing, and difficult-to-transfect cells, such as primary and stem cells, leading to long-term, stable expression of the transgene with low immunogenicity.
“With the LentiBrite™ pre-packaged lentiviral biosensors, researchers are able to easily and efficiently transfect cells and then visualize protein localization without disruption to cellular function,” describes Lucas Armstrong, Ph.D. R&D Manager of New Product Development. “Researchers can watch the real time movement of proteins involved in critical functions that are often altered in disease states such as cancer or induced through modulation.”
GFP-LC3--Catalogue No. 17-10193
GFP-LC3 Mutant--Catalogue No. 17-10189
GFP-Tubulin--Catalogue No. 17-10206
RFP-Tubulin--Catalogue No. 17-10205
GFP-Vimentin--Catalogue No. 17-10152
RFP-Vimentin--Catalogue No. 17-10153
GFP-Beta-actin--Catalogue No. 17-10204
RFP-Beta-actin--Catalogue No. 17-10203
For more information on the LentiBrite™ lentiviral biosensors, please visit www.millipore.com and search “Lentiviral Biosensor.”
DOWNLOAD VIDEO OF LIVE CELL IMAGING SHOWING PROTEINS TAGGED WITH LENTIBRITE BIOSENSORS http://www.youtube.com/watch?v=EPurQv9XvxI&feature=related
About EMD Millipore
EMD Millipore is the Life Science division of Merck KGaA of Germany and offers a broad range of innovative, performance products, services and business relationships that enable our customers' success in research, development and production of biotech and pharmaceutical drug therapies. Through dedicated collaboration on new scientific and engineering insights, and as one of the top three R&D investors in the Life Science Tools industry, EMD Millipore serves as a strategic partner to customers and helps advance the promise of life science.
Headquartered in Billerica, Massachusetts, the division has around 10,000 employees, operations in 67 countries and 2010 revenues of $2.2 billion. EMD Millipore is known as Merck Millipore outside of the U.S. and Canada.
Note: Merck KGaA or Merck shall mean Merck KGaA, Darmstadt, Germany