Testa Analytical Solutions sheds light on the benefits of using Dynamic Light Scattering (DLS) as a useful tool for aggregate screening. DLS is particularly useful in protein aggregate screening due to it being extremely sensitive to the larger compounds. Their NanoBrook 90Plus particle size analyzer utilizes DLS to reliably measure sub-micron samples.
Raffaele Carano, Applications Laboratory Manager, reiterated "Protein aggregation is a common challenge in the manufacturing of biological products. Protein scientists particularly like DLS because it provides them with a simple, fast and non-destructive test for aggregate detection, where the very same sample can also be used for Small-Angle X-ray Scattering (SAXS) or Small-Angle Neutron Scattering (SANS) experiments.” Continuing further, “SAXS or SANS are powerful techniques used to extract structural parameters and determine the overall structures and shapes of biological macromolecules, complexes, and assemblies in solution. The presence of aggregates is known to negatively affect SAXS and SANS results, consequently scientists can use DLS as a selection tool to make sure protein samples are “good” candidates for their measurements".