The new Mito-rOCR Assay Kit from Agilent Technologies is an easy and streamlined end-to-end solution that makes sophisticated analysis of mitochondrial function available to researchers of all skill levels. With this cost-effective and versatile kit, researchers can incorporate functional assessment of relative mitochondrial respiration into their cell physiology and disease pathology studies.
Mitochondria are central to energy production processes that drive cellular activity, and mitochondrial dysfunction is linked to numerous diseases and conditions. The Mito-rOCR assay enables more researchers to explore this crucial aspect of biology with accuracy and ease. The assay can be used with various compatible fluorescent plate readers and multimode imagers that meet its requirements for sensitivity, detection capabilities, and environmental control.
For example, the BioTek Cytation series of fluorescence plate readers and multimode imagers are ideal for the Mito-rOCR assay. Their advanced software allows them to handle various applications, maintain optimal conditions, and automate processes, making them reliable and adaptable for different research needs.
The Mito-rOCR assay measures cells’ relative oxygen consumption rate (rOCR), enabling sensitive detection of changes in mitochondrial function. This approach offers thorough insights into mitochondrial health and performance. With the Mito-rOCR Assay Kit, compounds are reconstituted directly in the assay medium, eliminating the need for additional solvents.
Notably, the assay is also reversible; the reagent remains outside the cells to prevent interference with cellular processes and can be easily removed to allow further downstream analyses. Data analysis is conducted using the Mito-rOCR Analysis Module within Agilent Seahorse Analytics. This cloud-based software makes importing data, linearizing signals, calculating slopes, and generating data tables and bar charts easy and streamlined.