Beckman Coulter Life Sciences has launched the CytoFLEX mosaic Spectral Detection Module, a modular solution for the spectral flow cytometry industry.
When connected to the CytoFLEX LX or S* Flow Cytometers, the CytoFLEX mosaic Spectral Detection Module delivers enhanced fluorescence sensitivity for dim and complex multicolor experiments and can detect nanoparticles as small as 80 nm. It uniquely provides two unmixing algorithms and up to 10 autofluorescence channels, with up to 88 detection channels. The richer data resulting from spectral analysis can help untangle the complexities of biological systems and diseases faster, with a nuanced understanding of immune cell subsets, their functions and interactions, thanks to simultaneously detecting multiple markers.
In contrast to conventional flow cytometry, spectral flow cytometry acquires the full emission spectrum of each antibody-associated fluorophore across all lasers, rather than measuring only the peak of emission. This powerful evolution delivers a more detailed and precise characterization of cellular properties to advance understanding of complex biological systems and diseases within each sample versus legacy approaches.
The CytoFLEX mosaic spectral detection module is powered by a unique algorithm capable of improving resolution compared to other available methods, along with unmixing accuracy checks to ensure efficiency. CytExpert software for spectral reduces the need for extensive training and can speed up the transition to spectral technology. Additionally, the module comes with tools to ease the full spectral workflow, with access to the FluoroFinder panel builder and a Cytobank premium license to provide all the tools researchers need to go from sample preparation to analysis of complex spectral data in an easy manner.
The new spectral module is available in two product configurations: CytoFLEX mosaic 88 for CytoFLEX LX Flow Cytometer and CytoFLEX mosaic 63 for 4-laser CytoFLEX S Flow Cytometer.