Bruker Corporation’s timsMetabo is a peak-performance 4D-Metabolomics mass spectrometer delivering unprecedented sensitivity, separation power and annotation confidence for small molecules, further enhanced by the novel TIMS ‘MoRE’ scan-mode. The timsMetabo system enables the generation of a “digital metabolome archive” for every sample, providing TIMS-enabled qual-quant performance with greatly increased confidence for automated annotation, leveraging 4D separations and reproducible molecular collision cross sections (CCS) at scale for 4D-Metabolomics and 4D-Lipidomics.
The benchtop timsMetabo system combines unique and powerful trapped ion mobility separation (TIMS) technology and time-focusing for exceptional sensitivity. Novel Mobility Range Enhancement (MoRE) acquisition leverages the enhanced ion capacity of the dual-stage TIMS-MX ion funnel, expanding its mass-to-charge and mobility ranges. Coupled with a new high-precision quadrupole and the innovative Athena Ion Processor (AIP), the timsMetabo is the next-generation 4D-Metabolomics research and validation workhorse for confidence in small molecule analysis in complex mixtures—with sensitivity at speed and scale.
The timsMetabo excels at resolving isomers, isobars, and interferences in routine operation owing to its unique additional real-time gas phase CCS ion separation capability, delivering highly-selective MS1-based quantification —at speed and scale.
For assays requiring precision at the limit of quantitation, the AIP-equipped timsMetabo provides cleaner, less-chimeric MS/MS acquisition at rates up to 300 Hz for targeted quantitative analysis using parallel reaction monitoring. The AIP leverages mobility information encoded by TIMS in each acquisition cycle for optimal transfer of MS and MS/MS-derived ions for TOF mass analysis.
These novel features synergistically produce a rich digital metabolome archive of metabolite identity and quantity from each sample, enabling confident analysis, and enabling AI in metabolomics with machine learning from large-scale, high-quality data sets. The timsMetabo supports both HESI and CSI ion sources for coupling with analytical flow, microflow and nanoflow high-performance liquid chromatography.