Beckman Coulter Life Sciences announces the launch of its new IR820 and IR870 infrared dye antibody conjugates. For the first time, research flow cytometry labs have a low noise solution, including single laser excitation, no dye interferences along with negligible overlap with other fluorochrome emissions, and ultralow autofluorescence background.
IR820 and IR870 reagents are the industry’s first directly conjugated antibodies for infrared excitation. The reagents are ideal for lineage marker staining, offering enhanced brightness to clearly identify respective gating antigens, while leaving detection of non-infrared dyes uncompromised due to near-to-complete absence of spectral overlap. Lineage gating markers now can be added, or if already existing in the antibody panel, moved far away from commonly used spectral ranges where typically the most precious dim signals reside.
Current flow cytometry fluorochrome conjugates do not cover the infrared range. Lineage markers used for identification of major subpopulations typically reside in the same spectral ranges as probes for weak co-expressed antigens, enabling characterization of subpopulations and their functional status. By this spectral colocalization, typically bright lineage markers may compromise resolution and sensitive detection of their dimmer co-expressed companions.