SoluLyse from Amsbio is a bacterial protein extraction reagent that provides a highly efficient yet gentle method for extracting soluble proteins from bacterial cells.
Based upon a proprietary non-ionic detergent formulation, with the SoluLyse reagent there is no need for time-consuming and cumbersome secondary treatment methods such as sonication or freeze thaw. The unique, mild SoluLyse formulation allows perforation of bacterial cell walls without denaturing proteins.
In addition, the SoluLyse reagent offers an up to ten-fold increase in the yield of soluble proteins when compared with other commercial lysis reagents.
Treatment of bacterial cells with SoluLyse results in rapid and efficient extraction of proteins that are suitable for affinity purification and analysis. The SoluLyse reagent may also be used for preparation of high purity inclusion bodies from total cell lysates.
Employing a simple and quick 1-step protocol, SoluLyse is proven to provide superior large-scale bacterial protein extraction and high-throughput cell lysis results. A recent study published in PNAS Nexus (OUP, 2025) highlighted SoluLyse as an effective reagent for high-throughput membrane protein research, demonstrating its performance in demanding applications. Broad compatibility with bacterial strains such as E. coli BL21 and affinity systems (GST, His), makes SoluLyse a plug-in reagent for protein-expression labs.