Joanna Plas

Joanna Plas

Senior Research Scientist

Articles by Joanna Plas

  • A Versatile Sample Mixer

    Thursday, April 20, 2023
    The Thermomixer comfort provides both mixing and temperature control simultaneously. We used it for various applications, from mixing samples for immunoblotting to antibody-beads conjugation. It reaches the desired temperature really fast and offers the option to set the desired time your samples ... read more

  • Detection of Secreted CXCL2 from LPS Treated Microglia Cells

    Tuesday, April 18, 2023
    The ELISA was used to analyse changes in the chemokine's level in conditioned media from LPS treated microglia. The aim was to study the impact of autotaxin inhibition (main source of LPA production) on LPS-induced secretion of proinflammatory chemokines. The kit provides you with enough reagents to... read more

  • Efficient Analysis of Secreted CXCL10 from Murine Microglia

    Wednesday, April 12, 2023
    The ELISA kit was utilized to study the secretion of CXCL10 from LPS-stimulated microglia cells and the impact of autotaxin inhibition on the accumulation of this chemokine. The kit works very well, providing reproducible results between experiments. The variation between replicates is also ... read more

  • Detection of CCL5 Secretion from Activated Microglia

    Tuesday, April 11, 2023
    We used the ELISA to study different chemokine secretion from LPS-stimulated BV-2 cells and the impact of autotaxin inhibition on extracellular accumulation of these analytes. The ELISA kit from Peprotech works extremely well for a non-precoated ELISA. Very cheap compared to a series of relevant ... read more

  • Cheap and Reliable ELISA for Quantification of IL1b Levels

    Friday, December 09, 2022
    Studies in experimental animal models revealed that systemic administration of LPS initiates a complex immunological response in the brain, resulting in microglial activation, priming and/or tolerance, memory deficits, and loss of brain synapses and neurons. The role of the ATX/LPA axis in ... read more

  • Great Antibody for Detection of Autotaxin Expression via Immunoblotting

    Thursday, December 08, 2022
    In isolated primary murine neonatal microglia and the murine BV-2 microglia cell line, exogenous LPA induces a pro-inflammatory phenotype, and many of these inflammatory reactions are attenuated in the presence of LPA5 antagonists. When BV-2 microglia are polarized with LPS, they increase ATX ... read more

  • Evaluation of IL6 Levels in Mouse Serum

    Wednesday, November 23, 2022
    The role of the ATX/LPA axis in inflammatory diseases is not entirely clear. In order to study the effects of inhibition of ATX and LPA5, we used different ELISAs to evaluate changes in the levels of proinflammatory cytokines in the serum of mice in the absence or presence of autotaxin and LPAR5 ... read more

  • A Reliable Primer for Detection of iNOS Expression

    Thursday, November 17, 2022
    Part of our study was to investigate whether ATX and LPA5 antagonism would affect LPS-induced neuroinflammation in vivo. Mice (n = 6–8 per group) were injected intraperitoneally (i.p.) with DMSO, LPS (5 mg/kg) plus DMSO, with or without PF8380 (‘PF’; 30 mg/kg) or AS2717638 (‘AS’; 10 mg/kg). After 24... read more

  • Easy Detection of PLD Activity but with Extreme Variations Between Replicates

    Monday, November 14, 2022
    In this assay, PLD hydrolyses phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric/fluorometric product, proportional to the PLD activity in the sample. Autotaxin belongs to the ENPP family of enzymes and displays phospholipase D (PLD) activity. ... read more

  • Fast and Reliable Detection of TNFa Levels Using the PeproTech ELISA Kit

    Wednesday, November 09, 2022
    The goal of our project was to investigate the role of the ATX/LPA/LPARs axis under inflammatory conditions. In order to study the effects of autotaxin and LPAR5 inhibition during systemic endotoxemia, we used different ELISAs to evaluate possible changes in the levels of proinflammatory ... read more
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