Scarlette CASSAL

Agar Plates with LB Broth (Miller)
Thursday, February 02, 2023
Very good microbial growth medium. I add antibiotics in it and use it for the selective cultivation of transformed E. coli. It is also possible to purchase agar and LB medium separately. It turned out to be less expensive. read more
Electrophoresis with Purple Gel Loading Dye
Thursday, January 26, 2023
I load my samples on agarose gels with this purple gel loading dye, and it works perfectly. It contains a dye that indicates the migration front, and the glycerol has been replaced by Ficoll, which gives tighter bands. read more
Bacteria Culture with LB Broth
Wednesday, January 25, 2023
I use this powder to make an E.coli growth medium. It works perfectly. The culture can then be used for mini or maxiprep, and also for protein purification. I sometimes use this medium for the recovery step after transformation by heat shock and it works too. read more
Monitoring NK Cells Activation with the Help of Monensin
Friday, January 20, 2023
I use monensin to follow the activation of NK cells when incubated with target cells. I add an anti-CD107a antibody at the beginning of the incubation, and then after 1h of incubation, I add monensin at 1/1000. It prevents the acidification of endocytic vesicles, protecting the reinternalized CD107a... read more
PCR with Q5 Hot Start High-Fidelity 2X Mix
Tuesday, January 10, 2023
This Q5 mix is really simple to use and allows the quick generation of PCR products without any mutation, which is really useful when the amplified DNA is used for cloning or sequencing. However, this mix is quite expensive. read more
Labelling NK Cells with Anti-CD56
Wednesday, January 04, 2023
I use the anti-CD56 antibody at a dilution of 1/100 to check the purity of my sample after performing a NK cell isolation from PBMC. I perform a 20-minute incubation at 4°C and then read the fluorescence by flow cytometry. I also use it to label NK cells among a pool of various cells that I have ... read more