Vincent Giacalone

Vincent Giacalone

Research scientist
I completed my PhD in immunology at Emory University focusing on innate immune responses in chronic respiratory diseases. I now work in the biotech industry developing assays to test immunomodulatory drug candidates. Select publications: https://pubmed.ncbi.nlm.nih.gov/35637404/ https://pubmed.ncbi.nlm.nih.gov/36445361/ https://pubmed.ncbi.nlm.nih.gov/32013006/

Articles by Vincent Giacalone

  • Reliable Intracellular Staining Kit

    Wednesday, August 16, 2023
    This buffer set from BD contains all the necessary solutions to perform fixation and permeabilization of cells in preparation for intracellular staining. After fixation, cells can be kept in the permeabilization buffer overnight to continue the staining the next day. This kit can be used to stain ... read more
  • Fast and Reliable Kit for Isolating Monocytes

    Wednesday, July 26, 2023
    Monocytes comprise only ~10% of circulating blood leukocytes, so obtaining sufficient number from blood can be a challenge due to low cell number. This kit can be used to purify monocytes from blood with no impact on cell viability or phenotype. It will not achieve complete removal of all other ... read more
  • Low-Binding Plates For Sample Storage

    Wednesday, June 21, 2023
    These 96 well plates are ideal for freezing cell pellets and supernatants. They have low binding to nucleic acids and protein to maximize sample recovery and are well tolerant of temperature change. One limitation of this product is that traditional plate seals do not stick well and plate lids are ... read more
  • Versatile Midi-size Staining System for Western Blot

    Wednesday, April 26, 2023
    This apparatus allows rapid staining of western blots similar to the standard iBind system, but can accommodate larger size blots and includes additional well attachments for staining 2 mini blots or 6 cut into separate strips. The primary and secondary antibodies are loaded at the same time, but ... read more
  • Convenient Smaller Size Conical Tube

    Monday, March 06, 2023
    15 mL tubes are well suited for centrifugation but can be cumbersome when working with smaller volumes in the 2-4 mL range. These 5 mL tubes are the same shape as standard 15 mL tubes but shorter to facilitate working with smaller volumes. This is especially useful if you are pelleting cells from 2-... read more
  • Versatile Adapter for Aspirator

    Monday, February 27, 2023
    This adapter is for connecting to the tubing of a vacuum aspirator setup. Instead of being limited to the standard aspirator pipette, this adapter allows the use of other attachments such as a standard pipette tip or even 8 at once to aspirate from wells of a 96 well plate. The is a rubber grommet ... read more
  • Compact and Reliable Benchtop Aspirating System

    Wednesday, February 15, 2023
    This aspirating system is a good setup for routine aspiration at the lab bench. This kit comes with the collection bottle and overflow bottle (both 1 L size), the secondary container, and tubing. I use it to aspirate culture media, supernatant from washing steps, and unused solutions, which I then ... read more
  • Large Freezing Chamber for Cryopreservation

    Friday, February 10, 2023
    The Freeze Cube can be used for transferring newly prepared cryovials of cells to -80°C for controlled freezing. I like that this chamber is able to accommodate taller 2 mL cryovials with the lid still fitting on. The capacity for 30 vials enables larger batches of freeze downs with only one chamber... read more
  • Digital Microscope for Cell Culture

    Monday, January 30, 2023
    This is a great microscope to use for viewing suspension or adherent cell cultures. The large real-time digital screen is much easier to view than staring through the eyepieces of a traditional microscope. This makes it much easier to show your cells to someone else, as you can connect a mouse and ... read more
  • Versatile Imaging System for Gels and Blots

    Friday, January 27, 2023
    This imager can perform fluorescent and chemiluminescent imaging of protein/DNA gels, western blots, and many other applications. The autofocus and auto exposure performance is quick and accurate, and there are several types of sample trays you can install depending on what you want to image. I like... read more
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