Cruz Meneses

Cruz Meneses

Postdoc

Articles by Cruz Meneses

  • Good Antibody for Cell Imaging

    Friday, September 03, 2021
    This antibody provides good data for cell imaging. 3T3 cells were fixed with 4% paraformaldehyde 15 min, permeabilized with 0.2% Triton X-100 in PBS for 10 min, blocked with 5% goat serum and 0.5% BSA in DPBS for 1 hour. Primary antibodies were added overnight at 4°C dilution 1:100. The samples were... read more

  • Semi-Dry Transfer Unit for WB

    Thursday, September 02, 2021
    Cells were rinsed with cold PBS and lysed in RIPA lysis buffer (0.1% NP40, 20 mM Tris/HCl pH 8, 10% Glycerol, with fresh protease inhibitors (Roche #04693132001) and phosphatase inhibitors (Calbiochem #524629). Cell lysates were cleared by centrifugation in a microcentrifuge (15,000 rpm for 10 ... read more

  • Great Antibody for Flow IF

    Wednesday, September 01, 2021
    3T3 cells were plated on glass coverslips and then directly transfected, or alternatively transfected with a later split onto coverslips. Cells were fixed with 4% paraformaldehyde 15 min, permeabilized with 0.2% Triton X-100 in PBS for 10 min, blocked with 5% goat serum and 0.5% BSA in DPBS for 1 ... read more

  • Easy to Use PCR Tubes Minifuge

    Tuesday, August 31, 2021
    The machine is small, practical and easy to use. Just push the button and you can stop anytime. Types of tubes that you can use: Spin PCR Tubes or Eppendorf tubes. You don't need a manual to learn how to use it. It's ideal for quick spins and filtration of samples, and it can be used also for a ... read more
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