HPLC Detector

An HPLC detector is used to analyze the components of the eluted mixture that is collected after being run through an HPLC column. Differences in detector type are based on what is being detected. Detection can be done by measuring absorbance, changes in reflex indices, molecular weight, and fluorescence among others. When selecting a detector, it is important to consider the sample type being analyzed. Some sample types will not work or will have lower sensitivity on particular detectors. Also of consideration is the type of information that is being sought by the analysis and what method will be used when running the column, for example, some detectors will work with the gradient method while others will not.

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CompanyWaters | Wyatt TechnologyWaters | Wyatt TechnologyWaters | Wyatt TechnologyWaters | Wyatt TechnologyWaters | Wyatt Technology
ItemomniDAWN™ Multi-angle Light Scattering (MALS) InstrumentCharged Aerosol Detector (CAD)DAWN™ Multi-angle Light Scattering (MALS) InstrumentmicroOptilab™ Refractive Index Detector for UHPLCOptilab™ Refractive Index Detector for HPLC/GPC
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Catalog NumberomniDAWNCADDAWNmicroOptilabOptilab
Price
TypeMulti-Angle Light Scattering (MALS) photometerInquireMALS and optional DLSRefractive Index DetectorRefractive Index Detector
Detector(s)InquireInquireHigh-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS.
Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength.
Differential Refractive Index (dRI)Differential Refractive Index (dRI)
ApplicationsWith standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio, and particle concentration. With the Calypso composition-gradient system: label-free characterization of biomolecular interactions, including self- and hetero-association as well as non-specific interactions.InquireWith standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration. With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions.Measure concentrations of all types of samples in almost all solventsGPC/SEC analysis of macromolecules by SEC-MALS
DescriptionDelivering up to 4× faster runs with half the sample, the omniDAWN™ multi-angle static light scattering (MALS) photometer is purpose-built for UPLC™ and UHPLC systems for non-destructive absolute molar mass and size characterization of macromolecules and nanoparticles in solution. It provides ... Read MoreThe Waters Charged Aerosol Detector (CAD) delivers reliable, near-universal detection that helps biopharmaceutical scientists quantify a wide variety of components involved in modern drug development. Its ability to provide a consistent response for molecules that lack strong UV absorbance makes it ... Read MoreThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass... Read MoreThe microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4... Read MoreThe Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same ... Read More
Wavelength RangeInquireInquireInquireInquireInquire
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