| Applications | Hydrophilic metabolites in metabolomics such as amino acids, nucleobases, nucleosides, nucleotides, coenzymes, and organic acids. | Basic Compounds & Peptides | Peptides, oligonucleotides, and biopharmaceuticals | Polysaccharides, proteins, biochemicals | Analysis of metal-coordinating and hydrophobic compounds such as nucleic acids, proteins, and lipids that adsorb to stainless steel and/or PEEK surfaces. |
| Description | The Shim-pack Mix-HILIC chromatography column for hydrophilic interactions enables separation of important amino acids in organisms as well as nucleobases, nucleosides, nucleotides, coenzymes, and organic acids via characteristic hydrophilic interactions and ionic interactions. Accordingly, it is The Shim-pack Mix-HILIC chromatography column for hydrophilic interactions enables separation of important amino acids in organisms as well as nucleobases, nucleosides, nucleotides, coenzymes, and organic acids via characteristic hydrophilic interactions and ionic interactions. Accordingly, it is suitable for the simultaneous monitoring of hydrophilic metabolites with a variety of physicochemical properties. The state of the functional group varies with pH. By utilizing two separation modes, anion-exchange and HILIC, a wide variety of components can be effectively separated and eluted. Recommended for polar compounds that have limited retention on alkyl or other HILIC phases.... Read More | Unprecedented Resolution and Peak Shape for High pH Compounds
Even for LC columns that claim to be designed for basic compounds, adequate resolution often cannot be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long Unprecedented Resolution and Peak Shape for High pH Compounds
Even for LC columns that claim to be designed for basic compounds, adequate resolution often cannot be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for high pH compounds.
Unmatched Peak Shape
Unmatched peak shape of basic compounds could be achieved while maintaining good peak shape for acidic compounds using the Shim-pack Arata LC column. Even when low ionic strength acidic mobile phase, such as 0.1% formic acid containing mobile phase are used, excellent peak shape of both amitriptyline (a basic compound) and benzoic acid (an acidic compound) could be achieved.
Excellent Separation Performance for Peptides Even with Weak Ion Pairing Acids
In order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS analysis. Excellent peak shape and separation performance for peptides can be achieved using the Shim-pack Arata LC column even with 0.1 % formic acid containing mobile phase.
Rapid Equilibration Even with Low Ionic Strength Acidic Mobile Phases
When analyzing basic compounds on a typical ODS column with low ionic strength acidic mobile phase, peak shape and long equilibration times are common problems. Shim-pack Arata LC columns can be rapidly equilibrated in low ionic strength acidic mobile phases yielding excellent peak shape and stable retention times.... Read More | Available at 2, 3, and 5 um particle sizes and ranging in length from 150 to 500 mm, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb, oligonucleotides and carbohydrates. By reducing the particle size from 5µm to 2µm, the resolution between Available at 2, 3, and 5 um particle sizes and ranging in length from 150 to 500 mm, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb, oligonucleotides and carbohydrates. By reducing the particle size from 5µm to 2µm, the resolution between aggregates and monomers was greatly improved. Furthermore, by reducing the column length from 300mm to 150mm using a 2µm particle, 50% less run time was achieved, while maintaining resolution as compared to the original method that used a 5µm, 4.6 × 300mm column.... Read More | Shim-pack Bio IEX Columns are available in Q (quaternary ammonium) and SP (sulfopropyl) chemistries and are based on porous (Q and SP columns) and non-porous (Q-NP and SP-NP columns) hydrophilic polymers with low nonspecific adsorption. The porous particles offer excellent binding capacity with Shim-pack Bio IEX Columns are available in Q (quaternary ammonium) and SP (sulfopropyl) chemistries and are based on porous (Q and SP columns) and non-porous (Q-NP and SP-NP columns) hydrophilic polymers with low nonspecific adsorption. The porous particles offer excellent binding capacity with exceptionally high efficiency and the non-porous particles offer high efficiency and exceptional resolution. Available in 3 or 5 um particle size and in lengths ranging from 30 to 100 mm.... Read More | Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.... Read More |