LC Columns / HPLC Column

Liquid chromatography (LC) columns, also known as high performance liquid chromatography (HPLC) columns, are used to separate the individual components of a sample for either purification purposes or for analysis. A liquid mobile phase is put through the HPLC column at a high pressure, and the separated components are eluted from the column at different times, allowing for the purified compounds to be put to another use or for the analysis of the chemical compounds in the sample. LC columns are used in the biomedical field to test for, among other things, blood alcohol levels, poisons in the body, and causes of death. Environmental sciences, clinical sciences, and many industries use the LC column to purify or find impurities in a substance. The type of high performance liquid chromatography column one uses depends on the type of sample that will be run through it. HPLC columns can be of different sizes and made of either glass or metal. LC columns can also have normal phase or reverse phase stationary phases.
CompanyYMC America, Inc.YMC America, Inc.YMC America, Inc.YMC America, Inc.
ItemYMC-Triart C18 HPLC ColumnYMC-BioPro IEX Ion Exchange HPLC ColumnYMC-Pack ODS-AQ C18 HPLC ColumnYMC-SEC MAB Size Exclusion HPLC Column
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DescriptionOrganic/inorganic hybrid silica C18 for reversed phase HPLCHydrophilic polymer-based ion exchange columns for protein purificationAqueous-compatible C18 reversed phase column for polar compound analysisSize exclusion chromatography column optimized for monoclonal antibody analysis
Mode of SeparationReversed PhaseIon ExchangeReversed PhaseSize Exclusion
Packing MaterialHybrid silica C18Porous and non-porous hydrophilic polymer beadsC18 (USP L1) with high carbon loadingDihydroxypropyl silica (USP L20, L59)
Pore Size120 AInquire120 A250 A
Particle Size1.9, 3, 5, 7, 10, 15, 20 um3, 5, 6, 10, 20, 30, 75 um3, 5, 10, 15, 20, 50 um3 um
End CappingMulti-stageInquireInquireInquire
ApplicationsAcidic, neutral, basic compounds, peptides, small molecules, APIsProtein purification, peptides, oligonucleotides, biomolecule separationHighly polar compounds, biomolecules, peptides, proteins, nucleotidesMonoclonal antibodies, aggregates, fragments, large proteins (10 kDa to 700 kDa)
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