LC Columns / HPLC Column

Liquid chromatography (LC) columns, also known as high performance liquid chromatography (HPLC) columns, are used to separate the individual components of a sample for either purification purposes or for analysis. A liquid mobile phase is put through the HPLC column at a high pressure, and the separated components are eluted from the column at different times, allowing for the purified compounds to be put to another use or for the analysis of the chemical compounds in the sample. LC columns are used in the biomedical field to test for, among other things, blood alcohol levels, poisons in the body, and causes of death. Environmental sciences, clinical sciences, and many industries use the LC column to purify or find impurities in a substance. The type of high performance liquid chromatography column one uses depends on the type of sample that will be run through it. HPLC columns can be of different sizes and made of either glass or metal. LC columns can also have normal phase or reverse phase stationary phases.
CompanyYMC America, Inc.YMC America, Inc.YMC America, Inc.YMC America, Inc.
ItemYMC-SEC MAB Size Exclusion HPLC ColumnYMC-BioPro IEX Ion Exchange HPLC ColumnYMC-Pack ODS-AQ C18 HPLC ColumnYMC-Triart C18 HPLC Column
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DescriptionSize exclusion chromatography column optimized for monoclonal antibody analysisHydrophilic polymer-based ion exchange columns for protein purificationAqueous-compatible C18 reversed phase column for polar compound analysisOrganic/inorganic hybrid silica C18 for reversed phase HPLC
Mode of SeparationSize ExclusionIon ExchangeReversed PhaseReversed Phase
Packing MaterialDihydroxypropyl silica (USP L20, L59)Porous and non-porous hydrophilic polymer beadsC18 (USP L1) with high carbon loadingHybrid silica C18
Pore Size250 AInquire120 A120 A
Particle Size3 um3, 5, 6, 10, 20, 30, 75 um3, 5, 10, 15, 20, 50 um1.9, 3, 5, 7, 10, 15, 20 um
End CappingInquireInquireInquireMulti-stage
ApplicationsMonoclonal antibodies, aggregates, fragments, large proteins (10 kDa to 700 kDa)Protein purification, peptides, oligonucleotides, biomolecule separationHighly polar compounds, biomolecules, peptides, proteins, nucleotidesAcidic, neutral, basic compounds, peptides, small molecules, APIs
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