LC Columns / HPLC Column

Liquid chromatography (LC) columns, also known as high performance liquid chromatography (HPLC) columns, are used to separate the individual components of a sample for either purification purposes or for analysis. A liquid mobile phase is put through the HPLC column at a high pressure, and the separated components are eluted from the column at different times, allowing for the purified compounds to be put to another use or for the analysis of the chemical compounds in the sample. LC columns are used in the biomedical field to test for, among other things, blood alcohol levels, poisons in the body, and causes of death. Environmental sciences, clinical sciences, and many industries use the LC column to purify or find impurities in a substance. The type of high performance liquid chromatography column one uses depends on the type of sample that will be run through it. HPLC columns can be of different sizes and made of either glass or metal. LC columns can also have normal phase or reverse phase stationary phases.
CompanyMacherey-Nagel Inc.Macherey-Nagel Inc.Macherey-Nagel Inc.Macherey-Nagel Inc.
ItemNUCLEOSHELL RP 18 Core-Shell HPLC ColumnNUCLEODUR C18 Isis HPLC ColumnNUCLEODUR HILIC HPLC ColumnNUCLEOSIL C18 Classic HPLC Column
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DescriptionCore-shell technology C18 column with enhanced pH stability and base deactivationHigh-purity silica C18 with polymeric surface for exceptional shape selectivityHILIC stationary phase for separation of highly polar compounds and ionic analytesClassic NUCLEOSIL C18 reversed phase column, original spherical silica developed in 1974
Mode of SeparationReversed PhaseReversed PhaseHILICReversed Phase
Packing MaterialOctadecyl (C18) core-shell superficially porous particlesNUCLEODUR C18 Isis - high purity silicaNUCLEODUR HILIC hydrophilic stationary phaseC18 (ODS) endcapped on NUCLEOSIL silica gel
Pore Size90 A110 A110 A100 A
Particle Size2.7 um3 um3 um5 um
End CappingYesYesNoYes
Flow RateInquire5 mL/min0.3 mL/minInquire
ApplicationsAnalgesics, antidepressants, drugs, herbicides, phytopharmaceuticalsAromatic compounds, fat-soluble vitamins, steroids, LC-MS suitableAmino acids, nucleosides, peptides, water-soluble vitamins, polar natural compoundsAldehydes, ketones, drugs, amino acids, vitamins, pesticides, phenolic compounds
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