LC Columns / HPLC Column

Liquid chromatography (LC) columns, also known as high performance liquid chromatography (HPLC) columns, are used to separate the individual components of a sample for either purification purposes or for analysis. A liquid mobile phase is put through the HPLC column at a high pressure, and the separated components are eluted from the column at different times, allowing for the purified compounds to be put to another use or for the analysis of the chemical compounds in the sample. LC columns are used in the biomedical field to test for, among other things, blood alcohol levels, poisons in the body, and causes of death. Environmental sciences, clinical sciences, and many industries use the LC column to purify or find impurities in a substance. The type of high performance liquid chromatography column one uses depends on the type of sample that will be run through it. HPLC columns can be of different sizes and made of either glass or metal. LC columns can also have normal phase or reverse phase stationary phases.
CompanyMacherey-Nagel Inc.Macherey-Nagel Inc.Macherey-Nagel Inc.Macherey-Nagel Inc.
ItemNUCLEOSIL C18 Classic HPLC ColumnNUCLEODUR C18 Isis HPLC ColumnNUCLEODUR HILIC HPLC ColumnNUCLEOSHELL RP 18 Core-Shell HPLC Column
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DescriptionClassic NUCLEOSIL C18 reversed phase column, original spherical silica developed in 1974High-purity silica C18 with polymeric surface for exceptional shape selectivityHILIC stationary phase for separation of highly polar compounds and ionic analytesCore-shell technology C18 column with enhanced pH stability and base deactivation
Mode of SeparationReversed PhaseReversed PhaseHILICReversed Phase
Packing MaterialC18 (ODS) endcapped on NUCLEOSIL silica gelNUCLEODUR C18 Isis - high purity silicaNUCLEODUR HILIC hydrophilic stationary phaseOctadecyl (C18) core-shell superficially porous particles
Pore Size100 A110 A110 A90 A
Particle Size5 um3 um3 um2.7 um
End CappingYesYesNoYes
Flow RateInquire5 mL/min0.3 mL/minInquire
ApplicationsAldehydes, ketones, drugs, amino acids, vitamins, pesticides, phenolic compoundsAromatic compounds, fat-soluble vitamins, steroids, LC-MS suitableAmino acids, nucleosides, peptides, water-soluble vitamins, polar natural compoundsAnalgesics, antidepressants, drugs, herbicides, phytopharmaceuticals
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