Polymerase chain reaction (PCR) is a workhorse technique of molecular biology laboratories in clinical and basic science. DNA thermal cyclers and gradient PCR units are essential in these laboratories. Selecting a good DNA thermal cycler is important. During PCR analysis, the well or tube capacity will determine the number of samples that can run at one time, including different combinations of temperature gradients (if available), MgCl2 concentrations, and primer levels within a single run. Temperature ranges, temperature ramp speeds, and programmability all influence an instrument’s versatility. PCR machines offer up to 384 well blocks, with temperatures reaching 100C. Some thermocyclers offer gradient capability and heated lids for more precise amplification protocols in PCR testing. The advantage of a PCR thermal cycler without gradient capability is low cost and applicability where thermal or concentration gradients are unnecessary.
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Analytik Jena
- 3–99°C (block control range)
- 96-well (0.2 mL) or 384-well or 60-well (0.5 mL); interchangeable block modules
- Yes, 30–110°C (HPSL technology)
- Yes (G models); up to 30°C differential across 12 rows (20–99°C range)
Analytik Jena
- 3–99°C
- 96-well (0.2 mL); accepts tubes, 8-well strips, or 96-well microplates
- Yes, 30–110°C (High-Performance Smart Lid, HPSL)
- Yes (TOne 96 G model only); semi-linear gradient, 20°C max differential over 12 columns (20–99°C range)
Analytik Jena
- 3 °C to 99 °C
- 96 and 384 well
- 30 °C–110 °C
- Yes, over 12 rows or 24 rows depending on model
Analytik Jena
- 4 °C to 99 °C
- 9 or 384 well
- 30 °C to 110 °C
- Linear Gradient Tool
Analytik Jena
- 4 °C to 99 °C
- 96 and 384 well models
- can be set to temperatures of up to 110 °C
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- Thermal Cycler Quality
Eppendorf North America
I used the Mastercycler Nexus X2 Thermal Cycler for the last 10 years for my research. I trust this thermal cycle because it provides highly reproducible results and is always reliable. I used the ... Read Review - Expensive but Worth It
Eppendorf North America
Was considering the Mastercycler X50 vs a couple other cheaper options. Ultimately went with the X50 and we don't regret it at all. It's super easy to use, and is very easy to maintain. I've always ... Read Review