DNA Thermal Cycler / PCR Instrument

Polymerase chain reaction (PCR) is a workhorse technique of molecular biology laboratories in clinical and basic science. DNA thermal cyclers and gradient PCR units are essential in these laboratories. Selecting a good DNA thermal cycler is important. During PCR analysis, the well or tube capacity will determine the number of samples that can run at one time, including different combinations of temperature gradients (if available), MgCl2 concentrations, and primer levels within a single run. Temperature ranges, temperature ramp speeds, and programmability all influence an instrument’s versatility. PCR machines offer up to 384 well blocks, with temperatures reaching 100C. Some thermocyclers offer gradient capability and heated lids for more precise amplification protocols in PCR testing. The advantage of a PCR thermal cycler without gradient capability is low cost and applicability where thermal or concentration gradients are unnecessary.
CompanyEppendorf North AmericaEppendorf North AmericaEppendorf North AmericaEppendorf North America
ItemMastercycler nexus GX2Mastercycler nexus X2Mastercycler® nexus Series Thermal CyclersMastercycler® X50 PCR Cycler
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Catalog Number63360000236337000027Mastercycler nexus6311000010
Price
Temperature Range4–99°C4–99°C4 to 99 °C4 °C to 99 °C
Capacity64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes (asymmetric dual block: 64-well + 32-well)64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes (asymmetric dual block: 64-well + 32-well)96 x 0.2 mL PCR tubes, 1 PCR plate 8 x 12 or up to 71 x 0.5 mL PCR tubes96- and 384-Wells
Heated LidYes – flexlid® technology with thermal sample protectionYes – flexlid® technology with thermal sample protectionYes, 37-110 °C temp. range37 °C–110 °C
Gradient CapabilityYes – 1–12°C gradient range over 8 columns (0.2 mL block); gradient temperature control range 30–99°CNo – no gradient block; gradient is only available on the GX2 variantYesYes
DescriptionInquireInquireThe Mastercycler® nexus can accommodate 96-well PCR plates, 0.2 mL PCR tubes, 0.2 mL PCR tube strips and 0.5 mL PCR tubes. This flexibility allows PCR users to select the consumables that best fit their assay. With less than 40 dB[a] noise emission, the nexus is whisper quiet, while also being ... Read MoreSpeed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology.

The excellent block temperature regulation gives rise to the next stage of PCR reproducibility, whereas the adaptable user management and
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