| Description | The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter workflows including multiplexed TR-FRET and AlphaScreen® assays, live-cell kinetic studies, and cytotoxicity assays. With over 85,000 scientific citations and four decades of trusted performance and partnership in research and regulated labs, the SpectraMax i-Series gives you the flexibility to adapt as your needs evolve. Whether you're accelerating discovery, ensuring QC consistency, or preparing for regulatory compliance, SpectraMax readers—paired with powerful, GxP-ready SoftMax® Pro Software—offer a future-ready platform that grows with your lab.
Reliable ELISA & Protein Quantitation- Perform sandwich and competitive ELISAs with high sensitivity
- Tunable monochromators and hybrid optics for assay flexibility
- Ideal for cytokine profiling, biomarker validation, and QC labs
Live-Cell & Cell Viability Assays- Support fluorescence and luminescence-based viability assays
- Optional CO₂/O₂ control and advanced shaking for live-cell workflows
- Suitable for cytotoxicity, proliferation, and pathway analysis
Multiplexed & Advanced Detection Assays- Perform TRF, fluorescence polarization, and multiplexed assays (iD5e/i3x)
- Expand into TR-FRET, HTRF®, and western blot detection as needs grow
- Reduce instrument sprawl while future-proofing your lab
... Read More | Dual monochromators increase performance and assay flexibility.
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm Dual monochromators increase performance and assay flexibility.
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station.
With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.
Patented optimization -
The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays.
IQ/OQ/PM Services -
Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.
Validation tools -
Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data.
Compliance software -
SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records.
Other features and benefits include: - - Assay flexibility
- Better absorbance accuracy
- Increased throughput
... Read More | Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.
Configuration changes in minutes -
Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.
Increased dynamic range and sensitivity -
Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.
Other features and benefits include: - Get automatic Z-height optimization
- Eliminate manual gain adjustment
- Tailor your data analysis
... Read More |
| Reading Modes | Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence (TRF), Fluorescence Polarization, TR-FRET, HTRF®, AlphaScreen® | Absorbance, Fluorescence, Luminescence,Time-Resolved Fluorescence, Fluorescence Polarization, HTRF | Endpoint reading, Kinetic reading, Well Area Scanning |