| Description | The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter workflows including multiplexed TR-FRET and AlphaScreen® assays, live-cell kinetic studies, and cytotoxicity assays. With over 85,000 scientific citations and four decades of trusted performance and partnership in research and regulated labs, the SpectraMax i-Series gives you the flexibility to adapt as your needs evolve. Whether you're accelerating discovery, ensuring QC consistency, or preparing for regulatory compliance, SpectraMax readers—paired with powerful, GxP-ready SoftMax® Pro Software—offer a future-ready platform that grows with your lab.
Reliable ELISA & Protein Quantitation- Perform sandwich and competitive ELISAs with high sensitivity
- Tunable monochromators and hybrid optics for assay flexibility
- Ideal for cytokine profiling, biomarker validation, and QC labs
Live-Cell & Cell Viability Assays- Support fluorescence and luminescence-based viability assays
- Optional CO₂/O₂ control and advanced shaking for live-cell workflows
- Suitable for cytotoxicity, proliferation, and pathway analysis
Multiplexed & Advanced Detection Assays- Perform TRF, fluorescence polarization, and multiplexed assays (iD5e/i3x)
- Expand into TR-FRET, HTRF®, and western blot detection as needs grow
- Reduce instrument sprawl while future-proofing your lab
... Read More | Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.
With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
User-configurable applications -
User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis -
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
Multi-channel functionality -
Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
Cellular pathway analysis -
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Other features and benefits include: - Capture flash assays with ease
- Reduce background noise
- Increase fluorescence performance
... Read More | The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on sensitivity or performance. Included SoftMax® Pro Software enables rapid data generation and analysis helping you achieve more in less time.
Features: - Industry-leading software - Enjoy an extensive library of protocols, advanced reader control, and easy data management and analysis with our best-in-class SoftMax Pro Software.
- Expanded wavelength range - Xenon flash lamps allow you to excite samples in the UV range where halogen does not.
- Approachable design - Effortless functionality provides users with everything they need to run their most crucial applications quickly and without complications.
- AutoPMT technology - Our AutoPMT feature allows a wide range of concentrations to be prepared and read on one plate, avoiding saturation (or the loss of signal) and saving you time and money.
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| Reading Modes | Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence (TRF), Fluorescence Polarization, TR-FRET, HTRF®, AlphaScreen® | Absorbance, Fluorescence (top/bottom read), Luminescence (top read), Time-Resolved Fluorescence (top read), Fluorescence Polarization (top read), HTRF (top read), AlphaScreen (top read) | UV-Vis absorbance, Fluorescence, and Luminescence |