| Description | The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter The SpectraMax® i Series of multi-mode microplate readers—iD3s, iD5e, and i3x—are designed to generate high-quality data for everyday assays such as DNA, RNA, and protein quantification, ELISAs, cell viability, and luciferase reporter assays, as well as complex, multi-parameter workflows including multiplexed TR-FRET and AlphaScreen® assays, live-cell kinetic studies, and cytotoxicity assays. With over 85,000 scientific citations and four decades of trusted performance and partnership in research and regulated labs, the SpectraMax i-Series gives you the flexibility to adapt as your needs evolve. Whether you're accelerating discovery, ensuring QC consistency, or preparing for regulatory compliance, SpectraMax readers—paired with powerful, GxP-ready SoftMax® Pro Software—offer a future-ready platform that grows with your lab.
Reliable ELISA & Protein Quantitation- Perform sandwich and competitive ELISAs with high sensitivity
- Tunable monochromators and hybrid optics for assay flexibility
- Ideal for cytokine profiling, biomarker validation, and QC labs
Live-Cell & Cell Viability Assays- Support fluorescence and luminescence-based viability assays
- Optional CO₂/O₂ control and advanced shaking for live-cell workflows
- Suitable for cytotoxicity, proliferation, and pathway analysis
Multiplexed & Advanced Detection Assays- Perform TRF, fluorescence polarization, and multiplexed assays (iD5e/i3x)
- Expand into TR-FRET, HTRF®, and western blot detection as needs grow
- Reduce instrument sprawl while future-proofing your lab
... Read More | Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.
With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
User-configurable applications -
User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis -
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
Multi-channel functionality -
Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
Cellular pathway analysis -
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Other features and benefits include: - Capture flash assays with ease
- Reduce background noise
- Increase fluorescence performance
... Read More | The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and temperature control, while the iD5e reader adds enhanced detection modes including time-resolved fluorescence (TRF) and tunable fluorescence polarization (FP) and can be expanded to include TR-FRET, HTRF, BRET and western blot. Both readers support on board fluidics with optional dual injectors.
For live-cell assays and microbial growth experiments, environmental control for CO2/O2 regulation and advanced shaking are available to maintain ideal growth conditions. Data acquisition and analysis are driven by SoftMax® Pro Software—the most cited microplate reader software in the industry.
Features:
- Supports diverse assay needs -
Perform ELISA, nucleic acid and protein quantitation, cell viability, microbial assays, western blot, and more.
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Simplified setup & analysis -
Over 200 preconfigured protocols and intuitive setup tools help streamline data acquisition and analysis—backed by the industry’s most cited microplate reader software.
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Flash Assays Icon -
Fast, consistent mixing for high-performance flash assays
Dual injectors available for fast kinetic assays with SmartInject® Technology, ensuring complete reagent mixing in every well for high-precision with 10 µL dead volume.
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Superior hybrid optical system-
The high-efficiency monochromator and filter-based optical system, combined with an ultra-cooled photomultiplier tube (PMT), minimizes noise while increasing sensitivity, selectivity, and dynamic range.
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Live cell assays-
Advanced shaking and user-installable gas mixer (CO2/O2) that support ideal environments for cell-based and microbial growth experiments are available as optional upgrades.
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Automatic filter identification-
Automatically identify filter sets using NFC functionality, simplifying workflows and ensuring accuracy and reliability.
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Flexible temperature control-
Precise and intuitive temperature regulation—from 5°C above ambient conditions up to 66 °C—empowering your lab to perform a wide range of temperature-sensitive assays with confidence.
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Read height optimization (Z height)-
Z-height optimization automatically adjusts the detection height to maximize sensitivity and signal quality—while minimizing sample volume—ensuring reliable results even in low-volume or precious samples.
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Microvolume & cuvette options-
The SpectraDrop Micro-Volume Microplate for low-volume (2 to 4 µL) and SpectraCuvette™ Adapter Plate for cuvette-based applications eliminate the need for additional instrumentation.
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Personalized touchscreen control-
The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.
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Western blot capable-
The optional enhanced time-resolved fluorescence (TRF) detection module is optimal for quantitating as little as femtogram protein samples, providing improved sensitivity, extended signal stability and versatility to support a wide range of applications.
... Read More |
| Reading Modes | Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence (TRF), Fluorescence Polarization, TR-FRET, HTRF®, AlphaScreen® | Absorbance, Fluorescence (top/bottom read), Luminescence (top read), Time-Resolved Fluorescence (top read), Fluorescence Polarization (top read), HTRF (top read), AlphaScreen (top read) | Absorbance, Fluorescence (top/bottom read), Luminescence (top/bottom read), Time-Resolved Fluorescence (top/bottom read), Fluorescence Polarization (top/bottom read), HTRF (top/bottom read), HTRF |