| Description | Automated pipetting ensures consistent performance over multiple assay conditions on a microplate.
The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling Automated pipetting ensures consistent performance over multiple assay conditions on a microplate.
The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics.
Onboard pipettor -
Integrated 8-channel or 16-channel pipettor increases assay flexibility by transferring reagents from 96 or 384 distinct wells in a source plate to the read plate.
Optimal assay excitation -
Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.
Low volume applications -
SpectraDrop™ Micro-Volume Microplate offers a high-throughput solution for low volume measurement, accelerating sample preparation time and increased productivity in DNA, RNA and protein research.
Robot integration -
The FlexStation 3 reader can be integrated with all leading partner robots and with our out-of-the-box automation solution for savings on up-front integration time and resources.
Other features and benefits include: - - Enable more assay conditions
- Automate pipetting
- Validate your reader
... Read More | Run everyday assays, explore new applications, and spend less time on setup and analysis. The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers bring absorbance, fluorescence, and luminescence detection together on one platform, with intuitive tools and optional capabilities that support Run everyday assays, explore new applications, and spend less time on setup and analysis. The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers bring absorbance, fluorescence, and luminescence detection together on one platform, with intuitive tools and optional capabilities that support your laboratory’s changing needs.
Choose the SpectraMax iD3s reader for core assay workflows, with a tunable monochromator that lets you select wavelengths to match your assays. For more advanced applications, the SpectraMax iD5e reader adds hybrid monochromator and filter-based optics, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP), with optional support for TR-FRET, including HTRF®, BRET, and western blot detection.
Both readers offer optional dual injectors for assays requiring reagent addition during measurement. Temperature control, multiple shaking modes, and an optional CO2/O2 gas mixer support live-cell and microbial growth experiments. SoftMax® Pro Software connects assay setup, data acquisition, and analysis, helping you turn measurements into interpretable results.
Features:
- Run multiple assay types on one platform – Perform ELISA, nucleic acid and protein quantitation, cell viability, and microbial assays using one reader and a common software platform. The iD5e reader supports additional applications, including optional western blot detection.
- Spend less time setting up and analyzing assays – Get started with more than 200 preconfigured protocols in SoftMax Pro Software. Intuitive setup and integrated analysis tools help reduce repetitive work and support consistent workflows across experiments.
- Support fast kinetic assays with consistent reagent mixing – Optional dual injectors with SmartInject® Technology deliver and mix reagents in the well for flash and other time-sensitive assays. Reverse Prime functionality helps recover unused reagent from injector tubing, reducing waste.
- Match detection settings to demanding assays – The iD5e reader’s hybrid optical system combines monochromator wavelength flexibility with filter-based sensitivity. An ultra-cooled photomultiplier tube helps reduce detector noise, supporting the measurement of weak signals.
- Control conditions during live-cell and microbial experiments – Multiple shaking modes and an optional, user-installable CO2/O2 gas mixer help you establish conditions suited to your cells or microorganisms during kinetic and longer-duration studies.
- Simplify filter setup when switching assays – On the iD5e reader, automatic filter identification uses NFC technology to recognize installed filters, making it easier to check the available filters when configuring an experiment.
- Maintain controlled temperatures throughout your assay – Temperature regulation from 5°C above ambient to 66°C supports temperature-sensitive reactions and growth studies, helping you maintain consistent measurement conditions.
- Optimize signal collection for your samples – Adjustable read height lets you optimize the position of the detection optics for your assay conditions, helping improve signal collection across different plate formats and sample volumes.
- Conserve precious samples and expand measurement formats – Measure samples in volumes of 2–4 µL with the optional SpectraDrop Micro-Volume Microplate, or use the SpectraCuvette™ Adapter Plate for compatible cuvette-based measurements on the same reader.
- Access routine workflows more quickly – Use touchscreen Quick Read icons to start common measurements and a personalized protocol library to access frequently used methods, simplifying setup for repeated experiments.
- Add western blot detection to your reader – Expand the iD5e reader with the optional enhanced TRF module to support western blot detection and low-abundance protein quantitation. Extended signal stability offers greater flexibility in detection timing, with sensitivity dependent on assay design and configuration.
... Read More | Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.
Eliminate manual gain adjustment -
The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.
Configuration changes in minutes -
Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.
Increased dynamic range and sensitivity -
Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.
Other features and benefits include: - Get automatic Z-height optimization
- Eliminate manual gain adjustment
- Tailor your data analysis
... Read More |
| Reading Modes | Absorbance Photometric, Fluorescence Intensity, Fluorescence Polarization, Time-resolved Fluorescence, Luminescence | Absorbance, Fluorescence (top/bottom read), Luminescence (top/bottom read), Time-Resolved Fluorescence (top/bottom read), Fluorescence Polarization (top/bottom read), HTRF (top/bottom read), HTRF | Endpoint reading, Kinetic reading, Well Area Scanning |